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pCMV2-hNCOA6:V5

Plasmid

#63585

Purpose

A subunit of the Trithorax-related (Trr) methyltransferase complex.

Depositor

Kenneth Irvine

Article

Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

Insert

NCOA6 (NCOA6 Human)

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
SBP-mCherry-Ecadherin_puromycin

Plasmid

#65293

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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ManII-SBP-EGFP_puromycin

Plasmid

#65260

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_flGalT-SBP-tagBFP

Plasmid

#65274

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pSR49

Plasmid

#69259

Purpose

phlh-8::tagBFP2

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

TagBFP2

Use

Cre/Lox

Tags

Expression

Worm

Mutation

codon-optimzed index 1.0, TagBFP2 contains 2 sing…

Promoter

hlh-8

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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ManII-SBP-mCherry_puromycin

Plasmid

#65261

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-tagBFP

Plasmid

#65257

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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HL 3722 (=HL 716 + pHL 404)

Plasmid

#30084

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3_3_TOPO_TA-ms2-VP64

Plasmid

#79371

Purpose

Recruits VP64 to a compatible gRNA for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 Y91F

Plasmid

#47093

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y91F (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Y91F

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
HL 839 (=HL 716 + pHL 108)

Plasmid

#30008

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:MicC-mCherry, pLtetO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
HL 2312 (=HL 716 + pHL 787)

Plasmid

#30024

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
HL 3858 (=HL 716 + pHL 1304)

Plasmid

#30088

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

mCherry, GFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
HL 2305 (=HL 716 + pHL 765)

Plasmid

#30020

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLtetO-1:DsrA, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
HL 2308 (=HL 716 + pHL 814)

Plasmid

#30022

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLtetO-1:OxyS, pLlacO-1:ompC::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
pNK875

Plasmid

#219735

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtTCTP

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter from Arabidopsis translationally controlled tumor protein (AtTCTP) gene

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

AtTCTP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK928

Plasmid

#219736

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter del5_MtHP

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter of MtHP gene from Medicago truncatula, del5_MtHP

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

del5_MtHP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK869

Plasmid

#219737

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

AtPD7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK1198

Plasmid

#219740

Purpose

MoClo-compatible Level 0 - 3U + terminator vector carrying terminator HSP18.2

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

The heat shock protein 18.2 (HSP) terminator

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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Nc-NylC_pET28a

Plasmid

#215412

Purpose

Expression construct for Nc-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Nc-NylC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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