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Plasmid#181790PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL0-NbPT5bPro
Plasmid#159416PurposeNicotiana benthamiana PT5b promoter sequence (1068 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertNbPT5b Promoter
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-NbBCP1bPro
Plasmid#159417PurposeNicotiana benthamiana BCP1b promoter sequence (1231 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertNbBCP1b Pro
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MjcDOPA5GT
Plasmid#162530PurposeMirabilis jalapa cyclo-DOPA 5-O-glucosyltransferase coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertcDOPA5GT
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD173 ZF43(AAAA)i
Plasmid#138833PurposeConstitutive expression of ZF43(AAAA)i under the CMV promoter.DepositorInsertZF43(AAAA)i
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR6A, R15A, R43A, R71APromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD389 VPR-ZF43(AAAA)
Plasmid#138826PurposeConstitutive expression of VPR-ZF43(AAAA) under the CMV promoter.DepositorInsertVPR-ZF43(AAAA)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR6A, R15A, R43A, R71APromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD388 VP64-ZF43(AAAA)
Plasmid#138822PurposeConstitutive expression of VP64-ZF43(AAAA) under the CMV promoter.DepositorInsertVP64-ZF43(AAAA)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR6A, R15A, R43A, R71APromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD113 VP16-ZF43(AAAA)
Plasmid#138818PurposeConstitutive expression of VP16-ZF43(AAAA) under the CMV promoter.DepositorInsertVP16-ZF43(AAAA)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR6A, R15A, R43A, R71APromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB004 CMV 3xFLAG-NLS-VP16-intN
Plasmid#161549PurposeConstitutive expression of 3xFLAG-NLS-VP16-intN under the CMV promoterDepositorInsert3xFLAG-NLS-VP16-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB005 CMV 3xFLAG-NLS-intC-ZF2
Plasmid#161550PurposeConstitutive expression of 3xFLAG-NLS-intC-ZF2 under the CMV promoterDepositorInsert3xFLAG-NLS-intC-ZF2
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA44
Plasmid#158522PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationL17E/D489VPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAM-B1
Plasmid#154056PurposeLevel 0 Golden Gate vector, contains domesticated NAM-B1DepositorInsertTaNAM-B1
UseSynthetic BiologyTagsExpressionBacterialMutationSynonymous mutation at base 3257, in exon 3, to r…PromoterN/AAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pZA18
Plasmid#158496PurposeIn planta gene expression of Golden gate compatibleA. thaliana ZAR1 D489V. Km plant selection marker.DepositorInsertZAR1 (ZAR1 Mustard Weed)
UseTagsExpressionPlantMutationD489VPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA36
Plasmid#158514PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA (ZAR1 Mustard Weed)
UseTags6xHAExpressionPlantMutationL17E, D489VPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-2
Plasmid#128651PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-1
Plasmid#128650PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA19
Plasmid#158497PurposeIn planta gene expression of Golden gate compatlible A. thaliana ZAR1 L17E. Km plant selection marker.DepositorInsertZAR1 (ZAR1 Mustard Weed)
UseTagsExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA35
Plasmid#158513PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA (ZAR1 Mustard Weed)
UseTags6xHAExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA33
Plasmid#158511PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA (ZAR1 Mustard Weed)
UseTags6xHAExpressionPlantMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only