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Showing: 4901 - 4950 of 156611 results

  3. pMT448

    Plasmid
    #139388
    Purpose
    Bile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1),
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    Jan. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    139388-plasmid-map-sequence-id-313834

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  4. pMT450

    Plasmid
    #139390
    Purpose
    Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    Jan. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    139390-plasmid-map-sequence-id-313840

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  5. pMT492

    Plasmid
    #139397
    Purpose
    Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    Jan. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    139397-plasmid-map-sequence-id-313938

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  7. TFORF2901

    Plasmid
    #144161
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DACH1 (DACH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144161-plasmid-map-sequence-id-396224

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  8. pAAV-DIO-CAG-TVA-P2A-dTomato

    Plasmid
    #177016
    Purpose
    For expression of the avian TVA receptor in a cre-dependent manner
    Depositor
    Peter Jonas
    Insert
    TVA-P2A-dTomato (CD320 Coturnix japonica)
    Use
    AAV and Cre/Lox
    Tags
    dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177016-plasmid-map-sequence-id-353739

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  10. Pet28a_Human_H3.1

    Plasmid
    #42631
    Depositor
    Joe Landry
    Insert
    Human H3.1 (H3C1 Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42631-plasmid-map-sequence-id-57746

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  15. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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  16. TFORF2902

    Plasmid
    #143658
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DACH1 (DACH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143658-plasmid-map-sequence-id-400440

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  20. pS-sg:GFP

    Plasmid
    #196296
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196296-plasmid-map-sequence-id-389821

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  21. pcDNA3 humanTRAF6 (1090)

    Plasmid
    #66930
    Purpose
    Mammalian Expression of TRAF6
    Depositor
    Michael Karin
    Insert
    TRAF6 (TRAF6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    66930-plasmid-map-sequence-id-190221

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  22. pCS2+mNeonGreen-C Cloning Vector

    Plasmid
    #128144
    Purpose
    To express a protein of interest fused to the C-terminus of mNeonGreen
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    mNeonGreen fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128144-plasmid-map-sequence-id-248605

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  25. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Article
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Expression
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

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  29. pMT493

    Plasmid
    #139398
    Purpose
    Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139398-plasmid-map-sequence-id-313939

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  30. pMT494

    Plasmid
    #139399
    Purpose
    Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139399-plasmid-map-sequence-id-313941

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  31. pMT449

    Plasmid
    #139389
    Purpose
    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139389-plasmid-map-sequence-id-313839

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  32. pMT451

    Plasmid
    #139391
    Purpose
    Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    BA inducible promoters
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139391-plasmid-map-sequence-id-313926

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  33. pMT455

    Plasmid
    #139392
    Purpose
    aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    aTC and BA inducible promoters
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139392-plasmid-map-sequence-id-313930

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  34. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Samuel Myers, Steven Carr
    Article
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    97421-plasmid-map-sequence-id-466895

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  37. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    92308-plasmid-map-sequence-id-216421

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  40. pDONR221-SLC22A3_STOP

    Plasmid
    #161190
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC22A3 (SLC22A3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161190-plasmid-map-sequence-id-333104

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  50. pVITRO1-Trastuzumab-IgG1/κ

    Plasmid
    #61883
    Purpose
    Expresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61883-plasmid-map-sequence-id-358014

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Showing: 4901 - 4950 of 156611 results