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3XFLAG-NSarQ27G-VMAn-FKBP

Plasmid

#70229

Purpose

Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

alpha Sarcin (N-terminal portion)

Use

Tags

3XFLAG, FK506- and rapamycin-binding protein (FKB…

Expression

Mammalian

Mutation

Changed Gln 27 to Gly

Promoter

CMV

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 L272A

Plasmid

#19850

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 L272A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 272 to Alanine

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 S449A

Plasmid

#19842

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1 S449A (MRTFA Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 449 to Alanine

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
EcPhnD90.L1∆∆AD.L297RL301R.pRSET

Plasmid

#33376

Purpose

a single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFP

Depositor

Loren Looger

Article

Alicea et al J Mol Biol. 2011 Oct 12.

Insert

phnD based Phosphonate Biosensor (phnD E. coli)

Use

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted in place of…

Promoter

T7

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCI-SMN2-inclusion-GLuc

Plasmid

#218671

Purpose

Alternative splicing signaling: signal increased with Ex7 inclusion increasing

Depositor

Jingxin Wang

Article

Tang et al Chembiochem. 2022 May 4;23(9):e202200012

Insert

SMN2 (SMN2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

Plasmid

#217665

Purpose

Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

OsTir1 F74G-SNAP-Blast-AAVS1

Use

Tags

SNAP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-SFFV_NKX2-1_Short_V5-GFP

Plasmid

#221500

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_CdTag-HA

Plasmid

#221493

Purpose

NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelCTerm_1-223AA

Plasmid

#221476

Purpose

Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelNTerm_160-371AA

Plasmid

#221475

Purpose

Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_HD_160-223AA

Plasmid

#221477

Purpose

Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelNTerm_160-371AA

Plasmid

#221487

Purpose

NKX2-1 N-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-hPGK_NKX2-1_Short_V5-GFP

Plasmid

#221498

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-UBC_NKX2-1_Short_V5-GFP

Plasmid

#221499

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Mutation

Promoter

UBC

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_Short_V5-GFP

Plasmid

#221497

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DmPGC

Plasmid

#220309

Purpose

red-light-activated guanylyl cyclase DmPGC

Depositor

Andreas Moeglich

Article

Xu et al J Mol Biol. 2024 Mar 1;436(5):168257. do

Insert

DmPGC

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDG04439

Plasmid

#216365

Purpose

xhNup133-Nb2t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup133-Nb2t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTP489

Plasmid

#216366

Purpose

xNup155-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup155-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC263

Plasmid

#216367

Purpose

xhNup155-Nb2i Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb2i

Use

Affinity Reagent/ Antibody

Tags

14xHis-Avi-SUMOEu1

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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