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Plasmid#221193PurposeGlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminatorDepositorInsertT7 promoter-glcO36
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoter with downstream glcO36 operatorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
SL3-MS2-gRNA GG backbone (MOBE3-4)
Plasmid#219949PurposeGolden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2DepositorInsertgRNA-MS2-SL3 (S. pyogenes Cas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
3'-com-gRNA GG backbone (MOBE1-4)
Plasmid#219947PurposeGolden gate backbone for mammalian expression of Sp-gRNA with 3'-comDepositorInsertgRNA-com-3'end (S. pyogenes Cas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
3'MS2-gRNA GG backbone (MOBE1-2)
Plasmid#219948PurposeGolden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2DepositorInsertgRNA-MS2-3'end (S. pyogenes Cas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1830
Plasmid#222728PurposepNP561 - T5 Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔNACs):GFP
Plasmid#218570PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationMutation genomic sequence 85nt, 86nt from TA to C…PromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL475
Plasmid#219503PurposeExpress Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in transDepositorInsertAde2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL2-160
Plasmid#219414PurposeEstradiol-inducible expression of 13-6 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-WT)-PDZLigWT-tSSA1}
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-TtrpC
Plasmid#213467PurposeVector containing the TrpC terminator for Golden Gate cloning in the Fg vectorDepositorInsertTerminator of tryptophan synthase of Aspergillus nidulans
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-PgpdA
Plasmid#213466PurposeVector containing the gpdA promoter for Golden Gate cloning in the Fg vectorDepositorInsertPromoter of the glyceraldehyde-3-phosphate dehydrogenase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-PtrpC
Plasmid#213465PurposeVector containing the TrpC promoter for Golden Gate cloning in the Fg vectorDepositorInsertPromoter of tryptophan synthase of Aspergillus nidulans
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGA-pCLV3
Plasmid#210528PurposeCloning A. thaliana CLV3 promoter as GreenGate module ADepositorInsertpCLV3 (CLV3 Mustard Weed)
UseSynthetic Biology; Golden gate compatible cloning…TagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Modular BE4max for GG insertion
Plasmid#198891PurposeEasily insert deaminase of choice into BE4max using Golden Gate cloningDepositorInsertCRISPR associated protein 9 (cas9 Streptococcus pyogenes)
UseTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNL2114 pARF19 GG0-GG1
Plasmid#195909PurposeLevel 0 pARF19 promoter with GG0 and GG1 golden gate linkersDepositorInsertpARF19 (ARF19 Mustard Weed)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I16 tUBQ1 GG5-GG6
Plasmid#195898PurposeLevel 0 tUBQ1 terminator with GG5 GG6 golden gate linkersDepositorInserttUBQ1 (UBQ1 Mustard Weed)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I15 tUBQ1 GG4-GG6
Plasmid#195897PurposeLevel 0 tUBQ1 terminator with GG4 GG6 golden gate linkersDepositorInserttUBQ1 (UBQ1 Mustard Weed)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)
Plasmid#178986PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)
Plasmid#178994PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)
Plasmid#178992PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)
Plasmid#178990PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only