Addgene

We narrowed to 27,415 results for: eng

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pHTN-HaloTag_ACTB:1-375

Plasmid

#210970

Purpose

Mammalian expression of full-length human ACTB. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

ACTB:M1-F375 (ACTB Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_CORO1A:1-461

Plasmid

#210964

Purpose

Mammalian expression of full-length human CORO1A. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1A:M1-K461 (CORO1A Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CORO1A:1-461

Plasmid

#210965

Purpose

Mammalian expression of full-length human CORO1A. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1A:M1-K461 (CORO1A Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_DCAF15:1-600

Plasmid

#210958

Purpose

Mammalian expression of full-length human DCAF15. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF15:M1-L600 (DCAF15 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_FBXW11:1-542

Plasmid

#210952

Purpose

Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW11:M1-R542 (FBXW11 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf1

Plasmid

#13676

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 (SMURF1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pBTK1017 (pNR)

Plasmid

#183131

Purpose

Control plasmid that does not express dsRNA

Depositor

Jeffrey Barrick

Article

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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GsI-IIC-RT

Plasmid

#181806

Purpose

GsI-IIC RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

GsI-IIC-RT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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MMLV-RT

Plasmid

#181801

Purpose

MMLV-RT (3XFLAG-SV40-MMLVRT)

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MMLV-RT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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PE-maturase-RT

Plasmid

#181807

Purpose

PE-E.r. maturase RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

PE-maturase-RT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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BBa_J72113-BBa_J72103

Plasmid

#40785

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi + cin + repL + pacA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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BBa_J72110-BBa_J72094

Plasmid

#40778

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pILGFP1F5

Plasmid

#165064

Purpose

Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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BBa_J72110-BBa_J72097

Plasmid

#40779

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS-pRhaB + Coi

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCT-L7.5.1

Plasmid

#42900

Depositor

Dane Wittrup

Article

Hackel et al J Mol Biol. 2008 Sep 19;381(5):1238-52.

Insert

L7.5.1

Use

Tags

HA and c-myc

Expression

Yeast

Mutation

Promoter

Gal1-10

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pTPTP alpha (Y789F)

Plasmid

#17693

Depositor

Xinmin Zheng

Article

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha Y789F (PTPRA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Y789F

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
maturase-RT

Plasmid

#181805

Purpose

E.r. maturase RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

maturase-RT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTPTP alpha (CCSS)

Plasmid

#17692

Depositor

Xinmin Zheng

Article

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha CCSS (PTPRA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C433S / C723S

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pRF032d

Plasmid

#156353

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Tags

Expression

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Vector 1174K

Plasmid

#221017

Purpose

Fluorescent based sex separator in Anopheles species mosquitoes (SEPARATOR)

Depositor

Omar Akbari

Insert

Engineered dobulesex splicing module (Anopheles gambiae)

Use

Synthetic Biology

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge