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Plasmid#166890PurposeFor vesicle exocytosis visualization using pHmScarletDepositorInsertVAMP2 (Vamp2 Mouse)
UseTagspHmScarletExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mCherry-T2A-Puro
Plasmid#72264PurposeExpress mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.DepositorInsertmCherry-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac PlayBack
Plasmid#203312PurposeTo all for PiggyBac gene insertion into GenomeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.3G
Plasmid#14748DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-Blast
Plasmid#83481PurposeLentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminaase -T2A -rtTADepositorInsertsSpCas9
Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
UseCRISPR and LentiviralTagsExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterTight Tre and human phosphoglycerate kinase 1 pro…Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRCaMP1a
Plasmid#61562PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRCaMP1a
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
attP-puro donor
Plasmid#182139PurposeattP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertionDepositorInsertattP-EGFP-EF1α-PuroR-T2A-TagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DogCatcher
Plasmid#171772PurposeExpresses DogCatcher protein in bacterial cytoplasmDepositorInsertDogCatcher
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP4B
Plasmid#116923Purposeexpresses mCherry-CHMP4B in mammalian cellsDepositorInsertCHMP4B (CHMP4B Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLKO.1-blast
Plasmid#26655Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_15bpLinker
Plasmid#157786PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_15bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0286
Plasmid#149333PurposeBacterial expression of SENP_EuB proteaseDepositorInsertSENP_EuB protease
UseTagsHis14 and Tev protease cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62
Plasmid#38277DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
mApple-TOMM20-N-10
Plasmid#54955PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 568, Emission: 592DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
gGBEv6.3-SpG
Plasmid#202630Purposevector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoterDepositorInsertnSpG(D10A)-MPGv6.3
UseTagsExpressionMammalianMutationSpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…PromoterhU6, EF1a, CBhAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pREP-8xARE-GFP-SV40-BFP
Plasmid#134910PurposeNRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFPDepositorInserts8xARE- minimal promoter - GFP
TagBFP
UseTagsExpressionMammalianMutationPromoter8xARE - minimal promoter and SV40Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP
Plasmid#160697PurposeExpresses GFP in mammalian cellsDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
STAT3-GFP
Plasmid#110495Purposereporter of STAT3 signaling activityDepositorInsert4 repeats of STAT3 binding site M67 at GFP promoter region
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[1-20]-N-int
Plasmid#199201PurposeGateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expressionDepositorInsertsplit-intein-Gal4[1-20]-N-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only