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Plasmid#181857PurposeGenetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-JNKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-AktAR
Plasmid#181856PurposeGenetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AktAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RABIF-FLAG
Plasmid#135558PurposeExpress human RABIF in mammalian cells, FLAG at N terminalDepositorInsertRABIF (RABIF Human)
UseTagsFlagExpressionInsectMutationPromoterT7 promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Q769gp120-8his
Plasmid#100929PurposeMammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strainDepositorInsertHIV-1 (Q769.d22) Env
UseTagsCD5 leader sequence and Purification tag after K5…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Dvl2
Plasmid#123587DepositorInsertdishevelled segment polarity protein 2 (Dvl2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR6-YFP
Plasmid#13020DepositorInsertToll-Like Receptor 6 (TLR6 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168
Plasmid#133976Purposemammalian expression vector of Flag tagged wild type RNF168DepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 Hexim2 (P#1897)
Plasmid#14663DepositorInsertHexim2 (HEXIM2 Human)
UseTagsHis and XpressExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-KLF5
Plasmid#40918DepositorInsertKruppel-like factor 5 (KLF5 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM4-Halo-FRB
Plasmid#197057PurposeExpression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM7-Halo-FRB
Plasmid#197058PurposeExpression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM22-Halo-FRB
Plasmid#197059PurposeExpression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Lrp6
Plasmid#123595DepositorInsertlow density lipoprotein receptor-related protein 6 (Lrp6 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα12-LgB115
Plasmid#134363PurposeNanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.DepositorInsertGα12-LgB115 (GNA12 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ash2L full
Plasmid#15548DepositorInsertAsh2L (ASH2L Human)
UseTagsExpressionMammalianMutationR622H (Please see depositor comments below)PromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
h-Plekhm1_FLAG-pcDNA 3.1
Plasmid#73593PurposeExpress human Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_FRB-GEM23-Halo
Plasmid#197060PurposeExpression of nanocage I3-01 Halo-FRB-fusion in mammalian cellsDepositorInsertI3-01
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mitoExRai-AKAR2
Plasmid#161757PurposeExRai-AKAR2 targeted to the outer mitochondrial membrane.DepositorInsertDAKAP-ExRai-AKAR2
UseTagsN-terminal 30 amino acids from DAKAP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_UnaG-Flag-Sec61B
Plasmid#83413PurposeExpressed N-term tagged UnaG on Sec61BDepositorInsertUnaG-Sec61B (SEC61B Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-Sumo1 Q94P
Plasmid#48965PurposeExpresses HA SUMO1 Q94P mutant in mammalian cellsDepositorInsertSUMO1 (SUMO1 Human)
UseTagsHAExpressionMammalianMutationMutated Q94PPromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only