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Plasmid#106256PurposeConstitutively expresses GFP fused with silaffin-R5 silica affinity peptideDepositorInsertGreen fluorescent protein
UseTagsAdded silaffin R5 peptide to C terminus of GFP af…ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-CAG-AAVS1
Plasmid#115262PurposeFor stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cellsDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPDS1-mEGFP-IRES-puro2b
Plasmid#26464DepositorInsertpPDS1 (PTTG1 Human)
UseTagsmEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo*
Plasmid#140159PurposeExpresses a regulatable secretory cargo (without an ER export signal) for mammalian cellsDepositorInsertpiGH-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
NSarQ27G-VMAn-FKBP
Plasmid#70230PurposeExpresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicingDepositorInsertalpha Sarcin (N-terminal portion)
UseTagsFK506- and rapamycin-binding protein (FKBP), Link…ExpressionMammalianMutationChanged Gln 27 to GlyPromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 S454A
Plasmid#19844DepositorInsertMKL1 S454A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 454 to AlaninePromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6 Baf60b
Plasmid#24723DepositorInsertBaf60b (Smarcd2 Mouse)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pZA-mCherry-R5
Plasmid#106258PurposeExpresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertmCherry
UseTagsAdded silaffin R5 peptide to C terminus of mCherr…ExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pRC3EGFP
Plasmid#193790PurposeExpresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPR promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
H2B-2A-GFP-IRESpuro2
Plasmid#183746PurposeExpresses H2B-2A-GFPDepositorInsertH2B (H2BC16P Human)
UseTagsGFP with 2A protease cleavage site between H2B an…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone
Plasmid#164266PurposesgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.DepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterU6 promoter from W05B2.8Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG5-mERRbeta-short
Plasmid#52188PurposeExpresses mouse ERRbeta short form splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-MKL1 D301-380
Plasmid#19852DepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 301 to 380PromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
p5E-pax3a-5.4k
Plasmid#164966Purposezebrafish pax3a -5.4k promoterDepositorInsertpax3a (pax3a Zebrafish)
UseGateway 5 end entry vectorTagsExpressionMutationpromoter -5.4kPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPTXLUC
Plasmid#68504Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter (AT1G33440 Mustard Weed)
UseTagsluciferaseExpressionBacterial and PlantMutationPromoterOPTXAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 T450A
Plasmid#19843DepositorInsertMKL1 T450A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Threonine 450 to AlaninePromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn1b
Plasmid#164957PurposeZebrafish kcnn1b gene CDSDepositorInsertkcnn1b (kcnn1b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-MKL1 D381-506
Plasmid#19853DepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 381 to 506PromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo*
Plasmid#140165PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogasterDepositorInsertssBip-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only