-
Plasmid#176930Purposeexpress Granulin 7 with linker 8 in mammalian cellsDepositorInsertGranulin 7+linker8 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 6-no linker
Plasmid#176929Purposeexpress Granulin 6 without linker 7 in mammalian cellsDepositorInsertGranulin 6 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 6+linker7
Plasmid#176928Purposeexpress Granulin 6 with linker 7 in mammalian cellsDepositorInsertGranulin 6+linker7 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 5-no linker
Plasmid#176927Purposeexpress Granulin 5 without linker 6 in mammalian cellsDepositorInsertGranulin 5 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 5+linker6
Plasmid#176926Purposeexpress Granulin 5 with linker 6 in mammalian cellsDepositorInsertGranulin 5+linker6 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 4-no linker
Plasmid#176925Purposeexpress Granulin 4 without linker 5 in mammalian cellsDepositorInsertGranulin 4 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 3-no linker
Plasmid#176923Purposeexpress Granulin 3 without linker 4 in mammalian cellsDepositorInsertGranulin 3 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 3+linker4
Plasmid#176922Purposeexpress Granulin 3 with linker 4 in mammalian cellsDepositorInsertGranulin 3+linker4 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 1-no linker
Plasmid#176919Purposeexpress Granulin 1 without linker 2 in mammalian cellsDepositorInsertGranulin 1 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-PP-human-CHMP3(150)
Plasmid#154181Purposeexpresses human CHMP3(150) in mammalian cellsDepositorInsertCHMP3 (CHMP3 Human)
UseTagsFLAG and Strep-Tag IIExpressionMammalianMutationC-terminal truncation of CHMP3 at amino acid 150PromoterCAGAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_TK1_HPRT1_Lb_6
Plasmid#155064PurposeLentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)DepositorInsert(hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_TK1_HPRT1_Lb_5
Plasmid#155063PurposeLentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a (CHyMErA system)DepositorInsert(hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_TK1_HPRT1_Lb_3
Plasmid#155061PurposeLentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system)DepositorInsert(hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_TK1_HPRT1_Lb_2
Plasmid#155060PurposeLentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)DepositorInsert(hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-SNCAe2-WT
Plasmid#85845PurposeDonor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFPDepositorInsertSNCA exon 2 homology arms (SNCA Human)
UseTags-ExpressionBacterial and MammalianMutation-PromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites
Plasmid#120295PurposeAAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sitesDepositorInsertC-terminal fragment of SpyCas9
UseAAV and CRISPRTagssplit-inteinExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-SNCAe3-A53T
Plasmid#85848PurposeDonor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFPDepositorInsertSNCA exon 3 homology arms (SNCA Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-α-Syn-nYFP
Plasmid#92203PurposeRetroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)DepositorInsertSNCA (SNCA Human)
UseRetroviralTagsYFP(1-155)ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW-hSNCA-GFP
Plasmid#215489PurposeLentiviral expression and easy visualization of alpha-synuclein in mammalian cellsDepositorInsertalpha-synuclein (SNCA Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterUbcAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
EIF2AK4 gRNA (BRDN0001146469)
Plasmid#75876Purpose3rd generation lentiviral gRNA plasmid targeting human EIF2AK4DepositorInsertEIF2AK4 (EIF2AK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only