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Showing: 4801 - 4850 of 148036 results

  5. pAAV-CAG-tdTomato (codon diversified)

    Plasmid
    #59462
    Purpose
    AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter
    Depositor
    Edward Boyden
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    codon diversified
    Promoter
    CAG
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    59462-plasmid-map-sequence-id-423489

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  9. Kaede-H2B-6

    Plasmid
    #57316
    Purpose
    Localization: Nucleus/Histones, Excitation: 508 / 572, Emission: 518 / 580
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    Kaede
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    57316-plasmid-map-sequence-id-256752

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  10. AICSDP-19:LAMP1-mEGFP

    Plasmid
    #101782
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1
    Depositor
    Allen Institute for Cell Science
    Insert
    LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101782-plasmid-map-sequence-id-198613

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  15. pBA904

    Plasmid
    #122238
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122238-plasmid-map-sequence-id-292011

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  23. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid
    #199252
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity eSpCas9(1.1)-D10A nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199252-plasmid-map-sequence-id-391383

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  27. mEGFP-C1

    Plasmid
    #54759
    Purpose
    Localization: C1 Cloning Vector, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54759-plasmid-map-sequence-id-320190

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  28. pAAV-hSyn-hChR2(H134R)-EYFP

    Plasmid
    #26973
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26973-plasmid-map-sequence-id-199313

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  29. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  30. mCherry-Lysosomes-20

    Plasmid
    #55073
    Purpose
    Localization: Lysosome Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55073-plasmid-map-sequence-id-215601

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  32. pAAV/L7-6-GFP-WPRE

    Plasmid
    #126462
    Purpose
    An AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.
    Depositor
    Hirokazu Hirai
    Article
    Nitta et al Mol Ther Methods Clin Dev. 2017 Jul 27;6
    Insert
    L7-6, Purkinje cell specific promoter
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    126462-plasmid-map-sequence-id-244965

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  33. IDG_TMC3_OE_1

    Plasmid
    #161629
    Purpose
    Overexpresses TMC3
    Depositor
    Mike McManus
    Insert
    TMC3 (TMC3 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    161629-plasmid-map-sequence-id-319361

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  34. Cx43-msfGFP

    Plasmid
    #69007
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Gja1 (Gja1 Rat)
    Use
    Tags
    monomerized superfolder GFP
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69007-plasmid-map-sequence-id-121423

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  38. pGEX6P1 METTL3

    Plasmid
    #200731
    Purpose
    Bacterial expression of GST-Tagged codon optimized human METTL3
    Depositor
    Claudio Alarcón
    Insert
    METTL3 (METTL3 Human)
    Use
    Tags
    GST from backbone plasmid
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200731-plasmid-map-sequence-id-401808

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  39. pAAV2/rh10

    Plasmid
    #112866
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap-rh10
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Truncated P5
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112866-plasmid-map-sequence-id-339914

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  46. AICSDP-25:MAP1LC3B-mEGFP

    Plasmid
    #101783
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B
    Depositor
    Allen Institute for Cell Science
    Insert
    MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101783-plasmid-map-sequence-id-197671

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  49. pcDNA3.1+(rInsp)-Ins-GLuc

    Plasmid
    #89928
    Purpose
    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    rat Insulin II promoter
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89928-plasmid-map-sequence-id-255702

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  50. CTNNB1 in pENTR

    Plasmid
    #216617
    Purpose
    gateway cloning donor vector containing CTNNB1
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    CTNNB1 (CTNNB1 Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216617-plasmid-map-sequence-id-427248

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Showing: 4801 - 4850 of 148036 results