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Showing: 961 - 970 of 148036 results

  1. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminaase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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  3. HK2

    Plasmid
    #25529
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HK2 (HK2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pXPR_047

    Plasmid
    #107145
    Purpose
    measures Cas9 activity
    Depositor
    David Root
    Insert
    GFP + sgRNA for GFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107145-plasmid-map-sequence-id-205295

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  5. TFORF3550

    Plasmid
    #145026
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145026-plasmid-map-sequence-id-422981

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  6. pGL3 Basic Vector

    Plasmid
    #212936
    Purpose
    Firefly luciferase vector for investigating regions controlling transcription
    Depositor
    Debrya Groskreutz
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212936-plasmid-map-sequence-id-419196

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  7. 3xnls-mScarlet-I

    Plasmid
    #98816
    Purpose
    In vivo visualization of the nucleus (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    3xNLS
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98816-plasmid-map-sequence-id-190172

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  10. pCutamp

    Plasmid
    #140632
    Purpose
    Plasmid-curing in Escherichia coli by targeting the AmpR promoter
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140632-plasmid-map-sequence-id-306006

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Showing: 961 - 970 of 148036 results