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Plasmid#121662PurposeU6-sgRNA EFS-ProCas9-FlaviS6-P2A-PuroDepositorInsertsUseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only
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F5A deltaCT S6K1 MSCV puro
Plasmid#8464DepositorInsertf5a delta ctp70 S6K1 (Rps6kb1 Rat)
UseRetroviralTagsmycExpressionMammalianMutationdeletion of 104 c-terminal amino acids mutation …PromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421)
Plasmid#170643PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-lacZ
Plasmid#72935PurposeLevel 1 Destination vector - Position ADepositorInsertLevel 1 - A site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCF710
Plasmid#121892PurposeEF1a-WNV-protease-mTagBFP2DepositorInsertsUseLentiviralTagsExpressionMutationPromoterEF1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcrF3
Plasmid#89246PurposePlasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).DepositorInsertAcrF3
UseTags6xHis-TEVExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA Raptor mutant 7
Plasmid#8475DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation738SLQN741-PAAA (S738P, L739A, Q740A, N741A)PromoterAvailable sinceMay 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
Mis12 INCENP Δcen mCherry
Plasmid#45228DepositorInsertMis12 INCENP Δcen mCherry
UseTagsVSVGExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
env8 selectivity (d')
Plasmid#99838PurposemNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.DepositorInsertenv8 selectivity (d')
UseTagsmNeonGreenExpressionBacterialMutationA20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…PromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-SAP25-126-186(pL91)
Plasmid#124387PurposeE.Coli produced SAP25 SID purified by FPLC nickel columnDepositorInsertGB1-His-SAP25-126-186 (SAP25 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-MKL1 D301-380
Plasmid#19852DepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 301 to 380PromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCF713
Plasmid#121665PurposeU6-sgRNA EF1a-ProCas9-FlaviS6-P2A-PuroDepositorInsertsUseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm2_A
Plasmid#145881PurposeInsect Expression of DmLSm2DepositorInsertDmLSm2 (CG10418 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm3_A
Plasmid#145882PurposeInsect Expression of DmLSm3DepositorInsertDmLSm3 (LSm3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm5_A
Plasmid#145884PurposeInsect Expression of DmLSm5DepositorInsertDmLSm5 (CG6610 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm7_A
Plasmid#145885PurposeInsect Expression of DmLSm7DepositorInsertDmLSm7 (LSm7 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat
Plasmid#101338PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tatDepositorInsertGFP, tat
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA Raptor mutant 2
Plasmid#8471DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation261DLF263-AAA (D261A, L262A, F263A)PromoterAvailable sinceMay 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJZ002
Plasmid#120231PurposeCRISPR-cas9 targeting in E. coli with ampicillin resistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA Raptor mutant 3
Plasmid#8473DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation313NWIF316-AAAA (N313A, W314A, I315A, F316A)PromoterAvailable sinceMay 11, 2005AvailabilityAcademic Institutions and Nonprofits only