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Plasmid#105423PurposeSynthetic biologyDepositorInsertNuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2
Plasmid#86593PurposeExpresses eGFP-tagged human Akt2(WT)DepositorInsertAkt2 (AKT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PM
Plasmid#197386PurposeTo constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).DepositorInsertPDX1-T2A-MAFA
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRV2-GG
Plasmid#105349Purposevirus-induced gene silencingDepositorInsertTRV-RNA2(5')
UseIn planta gene silencing; viralTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCherry
Plasmid#155222PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
2Flag-pDEST-N
Plasmid#118371PurposeGateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2FlagExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 Neo
Plasmid#127644PurposeEmpty pLentiCRISPRv2 with NeoRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minCMV
Plasmid#211518PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCitrine
Plasmid#155220PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
HLA_DRB1_04_01
Plasmid#165013PurposeExpression of HLA-DRB1*04:01 cDNADepositorInsertHLA-DRB1*04:01 (HLA-DRB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CD4-sTb(C)-M13-GFP
Plasmid#219780PurposesTb(C) half CaST for HEK cell expressionDepositorInsertCD4-sTb(C)-M13-GFP
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-Puro-2A
Plasmid#102611PurposeControl lentivector expressing Puro resistance and gene of interest from CMV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
HLA_DRB1_07_01
Plasmid#165014PurposeExpression of HLA-DRB1*07:01 cDNADepositorInsertHLA-DRB1*07:01 (HLA-DRB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRVL-2
Plasmid#104580PurposeContains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functionsDepositorInsertMouse IgG2c CH1/CH2/CH3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon
Plasmid#206218PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJOG641
Plasmid#105427PurposeSynthetic biologyDepositorInsertGAL4-VP16-GR (transcription factor: inducible expression; dexamethasone)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI042
Plasmid#105351PurposeSynthetic biologyDepositorInsertGal4UAS & 35S min promoter
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon hPGK
Plasmid#206230PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHBV-X/EnhI-Luc
Plasmid#71418PurposeTo see HBV X promoter(overlaped EnhanceI) activity for luciferase assayDepositorInsertHBV X promoter and enhancer I driving Luciferase expression
UseLuciferaseTagsExpressionMammalianMutationPromoterHBV XAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A
Plasmid#102585PurposeControl lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only