Addgene

We narrowed to 20,410 results for: crispr

Showing: 1901 - 1920 of 20410 results

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pTX168

Plasmid

#89257

Purpose

Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTX171

Plasmid

#89258

Purpose

Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTX172

Plasmid

#89259

Purpose

Express STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTX176

Plasmid

#89260

Purpose

Express estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTX179

Plasmid

#89261

Purpose

Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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SGL40C.EFS.dTomato

Plasmid

#89395

Purpose

Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pJB172

Plasmid

#86992

Purpose

CRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1

Depositor

Julien Berro

Article

Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

Insert

gRNA targeting pil1

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1- dCas9-2xNLS-EGFP

Plasmid

#74710

Purpose

Expresses dCas9-2xNLS-EGFP in mammalian cells

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

dCas9-2xNLS-EGFP

Use

CRISPR

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pMOD8A-iRGR-r11

Plasmid

#74369

Purpose

This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

Depositor

Eric Klavins

Article

Gander et al bioRxiv 041871

Insert

RGR-r11

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

AHD1

Availability

Academic Institutions and Nonprofits only

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pMOD8A-RGR-r10

Plasmid

#74370

Purpose

This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

Depositor

Eric Klavins

Article

Gander et al bioRxiv 041871

Insert

RGR-r10

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Availability

Academic Institutions and Nonprofits only

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pBLO1811_Cas9_noNLS_human

Plasmid

#74490

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBLO1811_arC9_noNLS_human

Plasmid

#74494

Purpose

Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

arC9

Use

CRISPR

Tags

t2a mCherry (C-terminal on insert)

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAN-OR-MalT-3NT

Plasmid

#62309

Purpose

synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
Malt-3NT sgRNA
A2NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PA2, PPhlF, and pBAD

Availability

Academic Institutions and Nonprofits only

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ptreCas9-mKate2ps-T1gRNA

Plasmid

#62715

Purpose

Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–T1gRNA

Plasmid

#62717

Purpose

t1 gRNA (ATGAGAATCAAGGCGGTCGA)

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–CgRNA

Plasmid

#64955

Purpose

Control gRNA (GTCAAGGCACTCTTGCCTA)

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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DR274-eGFP sgRNA

Plasmid

#61051

Purpose

for generation of a eGFP specific sgRNA from a T7 promoter

Depositor

Filippo Del Bene

Article

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Insert

EGFP sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

PcUBI4-2

Availability

Academic Institutions and Nonprofits only

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