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Showing: 1901 - 1920 of 150428 results

  2. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Use
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  4. pFA6a-link-ymScarletI-SpHis5

    Plasmid
    #219844
    Purpose
    tagging vector to tag genes with ymScarletI and a HIS5 gene
    Depositor
    Bas Teusink
    Insert
    ymScarletI-SpHis5
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 27, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    219844-plasmid-map-sequence-id-437596

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  7. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207411-plasmid-map-sequence-id-422696

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  8. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

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  11. pBsVchCAST

    Plasmid
    #203812
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Article
    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203812-plasmid-map-sequence-id-403828

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  12. TFORF2353

    Plasmid
    #144017
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF12 (ZNF12 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144017-plasmid-map-sequence-id-400529

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  13. pRDA174_EF1a_EnAsCas12a_BFP

    Plasmid
    #196724
    Purpose
    Sortable and selectable EnAsCas12a.
    Depositor
    Bernhard Schmierer
    Insert
    enASCas12a-HA-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS - HA and SV40 NLS
    Expression
    Mutation
    E174R, S542R, K548R
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196724-plasmid-map-sequence-id-388065

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  15. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

    Plasmid
    #195505
    Purpose
    T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
    Use
    CRISPR; Donor vector
    Tags
    mRuby-3xFLAG-NLS-3xSV40-poly(A)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195505-plasmid-map-sequence-id-384991

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  19. pAM5080

    Plasmid
    #85106
    Purpose
    Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85106-plasmid-map-sequence-id-189868

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Showing: 1901 - 1920 of 150428 results