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pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3h

Plasmid

#208699

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3h

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

Plasmid

#149276

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Tags

Expression

Mutation

T2318C (DNA) in PEBV coat protein sequence

Promoter

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.3G

Plasmid

#14748

Depositor

Christophe Benoist, Diane Mathis

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET42b-ABE7.10

Plasmid

#120398

Purpose

Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

Junjiu Huang

Article

Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

Insert

ABE7.10

Use

Tags

His

Expression

Bacterial

Mutation

Mammalian codon-optimized

Promoter

T7

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre

Plasmid

#13775

Purpose

Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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EZ-Tet-pLKO-Hygro

Plasmid

#85972

Purpose

Tet/Dox inducible shRNA lentivirus with hygromycin selection

Depositor

Cindy Miranti

Article

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

H1 / TetO

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-OSKM

Plasmid

#20321

Purpose

Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pJMP1356

Plasmid

#119276

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDIRECT_23C

Plasmid

#91140

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-SynB

Plasmid

#174861

Purpose

Expresses mouse syncytin B in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

syncytin B (Synb Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SFFV-DTAG-NTERM-GFP

Plasmid

#185760

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Expression

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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VAMP2-pHmScarlet

Plasmid

#166890

Purpose

For vesicle exocytosis visualization using pHmScarlet

Depositor

Pingyong Xu

Article

Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

Insert

VAMP2 (Vamp2 Mouse)

Use

Tags

pHmScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hTfR-HA

Plasmid

#69610

Purpose

human transferrin receptor with an HA tag fused at the C-terminus

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

human transferrin receptor (TFRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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phCMV-GALV-MTR

Plasmid

#163612

Purpose

Retro and Lentiviral transduction of primary human germinal center B cells

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Insert

GaLV MTR envelope

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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SFFV-IKZF3-CTERM-GFP

Plasmid

#185769

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Expression

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBT375

Plasmid

#125616

Purpose

Expresses evoAPOBEC1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ Grx1-roCherry

Plasmid

#141068

Purpose

Fluorescent biosensor for 2GSH/GSSG ratio

Depositor

Dmitry Bilan

Article

Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

Insert

Grx1-roCherry

Use

Tags

Expression

Mammalian

Mutation

Changed Alanine 150 to Cysteine, inserted Threon…

Promoter

CMV, SP6

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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TfR-sfGFP-myc tag-SpyCatcher003

Plasmid

#133451

Purpose

Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

TfR-sfGFP-myc tag-SpyCatcher003

Use

Tags

GSSGS and myc tag

Expression

Mammalian

Mutation

Contains C20 and A23 mutations that improve plasm…

Promoter

CMV

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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LV-TRE-WT human MyoD-T2A-dsRedExpress2

Plasmid

#60628

Purpose

Expresses WT human MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 mouse MyoD (MYOD1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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