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CCKBR-Tango

Plasmid

#66237

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CCKBR (CCKBR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pBSM

Plasmid

#67505

Purpose

Express the tRNAMet scaffold

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet

Use

Tags

Expression

Bacterial

Mutation

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-beta-3

Plasmid

#27289

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin beta 3 (ITGB3 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

n/a

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pAS1NB c Rosella I

Plasmid

#71245

Purpose

a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

Depositor

Mark Prescott

Article

Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

Insert

Rosella

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

Plasmid

#174515

Purpose

Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

Mm PylS
MmtRNA-Pyl-opt(UGA)

Use

Tags

Expression

Bacterial

Mutation

ASL from the serT gene (UGA anticodon) and recoded

Promoter

GlnS and lpp

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-stGtACR1(FusionRed)-W3SL

Plasmid

#218761

Purpose

Provides AND intersectional (Cre+Flp) expression of stGtACR1-FusionRed

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

stGtACR1

Use

AAV

Tags

FusionRed

Expression

Mutation

Promoter

hSyn

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX_lenti_osTir1_9Myc_P2A_Bsd

Plasmid

#139746

Purpose

lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

Depositor

Robert Tjian

Article

Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

Insert

rice E3 ligase Tir1 (osTir1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENV8(WT)-GFPuv

Plasmid

#70127

Purpose

Cobalamin riboswitch reporter vector for use in E. coli

Depositor

Robert Batey

Article

Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

Insert

wild type environmental cobalamin riboswitch sequence (env8)

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMIN-Ri

Plasmid

#197784

Purpose

Level 2 destination vector: pMIN-Ri backbone

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV18 L1L2

Plasmid

#52592

Purpose

Expresses HPV18 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV18 L1
HPV18 L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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