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Plasmid#86884PurposeBacterial expression of E. coli SRAP ortholog YedKDepositorInsertYedK (yedK Eschericia coli)
UseTags6xHis, HA tag, and S-TagExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgRNA AAVS1
Plasmid#128119PurposeCo-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA AAVS1 + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-RFP
Plasmid#72940PurposeLevel 1 Destination vector - Position BDepositorInsertLevel 1 - B site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-HA-mMcl-1
Plasmid#25385DepositorInsertmMCL1 (Mcl1 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NRASlong
Plasmid#13797DepositorInsertNRAS (NRAS Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
JP1520 PIK3CA WT HA
Plasmid#14570DepositorInsertPIK3CA (PIK3CA )
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pXM03
Plasmid#121150PurposeThis plamid was used for biosynthesis of Prenylated 4‑Hydroxybenzoate in E.coliDepositorInsertXimB, IDS2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSDMA66
Plasmid#128355PurposegRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.DepositorInsertNoureothricin (NAT)
UseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV-
Plasmid#101337PurposeRepaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.DepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-Sin3a-115-212(pL01)
Plasmid#124386PurposeE.Coli produced SIN3A PAH1 purified by FPLC nickel columnDepositorInsertHis-Sin3a-115-212 (SIN3A )
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA Raptor mutant 1
Plasmid#8470DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation194YDC196-AAA (Y194A, D195A, C196A)PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBP-lldR
Plasmid#74095PurposeL-lactate repressor (LldR) from E. coli MG1655DepositorInsertlldR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSDMA67
Plasmid#128356PurposegRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.DepositorInsertNoureothricin (NAT)
UseUnspecifiedTagsExpressionMutationPromoterACT1Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-Sestrin2-E451A
Plasmid#73677PurposeoverexpressionDepositorInsertSesn2 (SESN2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationE451A changed Glutamate 451 to AlaninePromoterCMVAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV rev
Plasmid#101339PurposeLTR drives GFP and internal SFFV promoter drives codon optimized revDepositorInsertGFP, rev
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-GFPγ-SpHis5
Plasmid#60781PurposeYeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selectionDepositorInsertGFPγ
UseYeast integratingTagsExpressionMutationT65C S72A V163A I167T L231HPromoterAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cherry-tTA-ER-C3
Plasmid#134567PurposeExpresses a Cherry-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion proteinDepositorInserttTA-ER
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-RFP
Plasmid#72939PurposeLevel 1 Destination vector - Position ADepositorInsertLevel 1 - A site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA Raptor mutant 4
Plasmid#8474DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation391SQ392-PA (S391P, Q392A)PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NRASshort
Plasmid#13799DepositorInsertNRAS (NRAS Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only