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Plasmid#49267Purposeretroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequenceDepositorInsertAkt (AKT1 Bovine)
UseRetroviralTagsIRES-EGFP and myrExpressionMammalianMutationN2D, D149E and A478G compared to AAW71957.1PromoterAvailable sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla 3A
Plasmid#84872PurposeBacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
Fla
UseTagsHisExpressionBacterialMutation3 C-terminal leucines have been replaced by alani…PromoterT7Available sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + FlaAAA
Plasmid#84867PurposeBaculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
FlaAAA
UseBaculovirusTags6xHisExpressionInsectMutation3 C-terminal leucines have been replaced by alani…PromoterPolyhedrinAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-cdc42palm
Plasmid#20141DepositorInsertcell division cycle 42 isoform 2 (CDC42 Human)
UseTagsGFPExpressionMammalianMutationwild typePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-cdc42prenyl
Plasmid#20142DepositorInsertcell division cycle 42 isoform 1 (CDC42 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + PrgJ
Plasmid#84869PurposeBaculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
PrgJ
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBK1302-AAV-EFSNC-dCjCas9-KRAB
Plasmid#223147PurposeExpression of KRAB with dCjCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1312-AAV-EFSNC-dSaCas9-KRAB
Plasmid#223157PurposeExpression of KRAB with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9
Plasmid#223163Purpose2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2G2A
Plasmid#174334Purposeencodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationmutation of a.a 2 Gly to AlaPromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2EFMUT
Plasmid#174333Purposeencodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationNKD2132DFD134>AFA (substitutions of D132 and D…PromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-GFP
Plasmid#129092PurposeKI EGFP with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertEGFP and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag‐hTim1
Plasmid#49207Purposeexpresses Flag-tagged human Tim1DepositorInsertTim1 (HAVCR1 Human)
UseTagsFLAG and signal sequenceExpressionMammalianMutationTim1 lacks its own signal sequencePromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmReNL
Plasmid#182970PurposeStably express PalmReNL in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmReNL
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-CreER
Plasmid#129093PurposeKI CreER with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertCreERT2 and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmNanoLuc
Plasmid#182969PurposeStably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmNanoLuc
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT6
Plasmid#164697PurposeExpression of PRMT6 with an N-terminal FLAG tagDepositorInsertPRMT6 (PRMT6 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-hCRACR2A-isoform-a
Plasmid#79593PurposeTransient expressionDepositorInsertCRACR2A-isoform-a (CRACR2A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3
Plasmid#49208Purposeexpresses Flag tagged mouse Tim3DepositorInsertTim3 (Havcr2 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationno Tim3 signal sequencePromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim1 (Bl/6)
Plasmid#49205Purposeexpresses Flag tagged mouse Tim1DepositorInsertTim1 (BL/6 allele) (Havcr1 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationTim1 lacks its own signal sequencePromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only