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Showing: 4701 - 4750 of 156611 results

  9. pLIX403-ccdB-G418

    Plasmid
    #158561
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158561-plasmid-map-sequence-id-309877

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  10. pLVX-Tet3G blasticidin

    Plasmid
    #128061
    Purpose
    Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128061-plasmid-map-sequence-id-255115

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  16. VIPR1-Tango

    Plasmid
    #66520
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    VIPR1 (VIPR1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. PGAM5

    Plasmid
    #38976
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PGAM5 (PGAM5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. TFORF1816

    Plasmid
    #143980
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SATB1 (SATB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143980-plasmid-map-sequence-id-400539

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  21. mCherry-TOMM20-N-10

    Plasmid
    #55146
    Purpose
    Localization: Outer Mitochondrial Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55146-plasmid-map-sequence-id-334999

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  22. R777-E293 Hs.RPS6KA3

    Plasmid
    #70577
    Purpose
    Gateway ORF clone of human RPS6KA3 [NM_004586.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KA3 (RPS6KA3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70577-plasmid-map-sequence-id-181747

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  24. pACEC3GH (Hyg)

    Plasmid
    #110084
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110084-plasmid-map-sequence-id-211189

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  31. pAAV MP-84 mRFP

    Plasmid
    #206926
    Purpose
    The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.
    Depositor
    Markus Grompe
    Article
    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2
    Insert
    mRFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MP-84
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206926-plasmid-map-sequence-id-407770

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  35. TFORF0770

    Plasmid
    #141693
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MSX2 (MSX2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141693-plasmid-map-sequence-id-408934

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  39. LRP8-bio-His

    Plasmid
    #51879
    Purpose
    Expresses full-length Low-density lipoprotein receptor-related protein 8 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    LRP8 (LRP8 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51879-plasmid-map-sequence-id-79841

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  40. pPEPY-PF6-lacI

    Plasmid
    #85589
    Purpose
    Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do
    Insert
    lacI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PF6
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    85589-plasmid-map-sequence-id-172946

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  43. AbVec2.0-IGHG4

    Plasmid
    #99578
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    99578-plasmid-map-sequence-id-192595

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  44. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid
    #225756
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Article
    Walton et al bioRxiv 2024.03.17.585235
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225756-plasmid-map-sequence-id-446064

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  45. LbCas12a-niCPE

    Plasmid
    #213747
    Purpose
    For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Insert
    MCP-nLbCas12a-M-MLV RT∆RNase H
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R, R1138A in LbCas12a
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213747-plasmid-map-sequence-id-422190

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Showing: 4701 - 4750 of 156611 results