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Plasmid#119916PurposeMammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARδ
UseTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARε
Plasmid#119917PurposeMammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARε
UseTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMSCV_IRES_GFP_CD16a
Plasmid#196189PurposeRetroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.DepositorInsertCD16a (FCGR3A Human)
UseRetroviralTagsExpressionMammalianMutationchanged Phenylalanine 158 to ValinePromoterLTRAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_MMP-9-HA
Plasmid#121172PurposeExpression of mouse MMP9DepositorInsertMMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
UseTagsHAExpressionMammalianMutationSee depositor comments belowPromoterCMV immediate/earlyAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ERBB2-V5
Plasmid#214612PurposeERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment assayDepositorInsertERBB2 (ERBB2 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 RPEL1-EGFP-3XNLS
Plasmid#58469PurposeExpresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoterDepositorInsertsUseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDG459
Plasmid#100901PurposeSpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-PuroRExpressionMammalianMutationPromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)
Plasmid#101139PurposeLuciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetOn-Cdt1-mCherry
Plasmid#193760PurposeDoxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag)DepositorInsertCdt1-mCherry (CDT1 Human)
UseLentiviralTagsmCherryExpressionMutationWTPromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
LcV2-Hygro
Plasmid#91977PurposeLentiCRISPR v2 with Hygro cloned in place of PuroDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-BoxB
Plasmid#92004PurposeExpresses firefly luciferase with BoxB sequences in 3' UTRDepositorInsertFirefly Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-iCasp9
Plasmid#199441PurposeExpress inducible Caspase9 in mammalian cellsDepositorInsertinducible Caspase9 (CASP9 Human)
UseLentiviralTagsDmrB and mCherryExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FLAG-METTL3
Plasmid#160250PurposeExpress FLAG-METTL3 in human cellsDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_3E5E_eGFP
Plasmid#103054PurposeEncodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoterDepositorInsertEbola virus (ebolavirus Zaire, Mayinga) minigenome
UseTags3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…ExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-synaptophysin-Lime
Plasmid#201016PurposeExpresses Lime in synaptic vesicle lumen by fusion to synaptophysinDepositorInsertSynaptophysin-Lime (Syp Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-OSH2-2xPH
Plasmid#161987PurposeMammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFPDepositorInsertOSH2 (OSH2 Budding Yeast)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pArk313
Plasmid#200257PurposeArk313 rep/capDepositorInsertArk313 Rep/Cap
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-BioID-FAM21
Plasmid#121046PurposeExpresses GFP tagged BioID and human FAM21C in mammalian cellsDepositorInsertsUseTagsGFPExpressionMammalianMutationR118G (highly promiscuous form)PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/V5-DEST-GFP
Plasmid#40125DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrispr-V2-mOrange (V2mO)
Plasmid#140206PurposeLenti Crispr/Cas9 with mOrange fluorescent markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterpELF1aAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3xFLAG-GFP-puroR
Plasmid#167463Purpose3rd-gen lentiviral expression of FLAG-GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDG458
Plasmid#100900PurposeSpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAGExpressionMammalianMutationPromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-Rbfox2
Plasmid#213726PurposeMammalian cell expression vector for overexpression of Rbfox2 in motor neurons.DepositorInsertRbfox2 (Rbfox2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
C1A-C1A-EYFP
Plasmid#61155PurposeDiacylglycerol (DAG) reporterDepositorInsertC1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
UseTagsEYFPExpressionMammalianMutationC1A from PKCG amino acids 26-89PromoterCMVAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-Rbfox1
Plasmid#213725PurposeMammalian cell expression vector for overexpression of Rbfox1 in motor neurons.DepositorInsertRbfox1 (Rbfox1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-Rbfox3
Plasmid#213727PurposeMammalian cell expression vector for overexpression of Rbfox3 in motor neurons.DepositorInsertRbfox3 (Rbfox3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
UseTagsExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EGFP
Plasmid#91975PurposeEGFP reporter cell line control (no miR-19 sites)DepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKESaPar
Plasmid#211829PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFGAMS-mEos2
Plasmid#105128PurposeExpresses human FGAMS-mEos2 fusion in mammalian cellsDepositorInsertPFAS (PFAS Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV PGK1-3xHA
Plasmid#220913PurposeExpresses PGK1-3xHADepositorInsertPGK1-3xHA (PGK1 Human)
UseAffinity Reagent/ AntibodyTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(-30)GFP
Plasmid#62936PurposeExpression of 6xHis-(-30)GFP in bacterial cellsDepositorInsert(-30)GFP
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
BK alpha subunit
Plasmid#113566Purposemouse BK channel alpha subunit expression vectorDepositorInsertKcnma1 (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos36)GFP
Plasmid#62937PurposeExpression of 6xHis-(+36)GFP in bacterial cellsDepositorInsert(pos36)GFP
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-eGFP-Cre
Plasmid#120219PurposeExpresses cre under the control of CamKIIa promotorDepositorInsertcre
UseAAV and Cre/LoxTagsEGFPExpressionMutationPromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Flag-Tet1 CD
Plasmid#70129PurposeExpress flag-tagged mouse Tet1 catalytic domain.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain.PromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_Lambda-N-HA-Peptide
Plasmid#92005PurposeExpresses Lambda-N-HA peptideDepositorInsertLambda-N-HA peptide
UseTagsLambda-N-HAExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only