Addgene

153,597 results

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pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
AAV2-hSyn-G-Flamp1

Plasmid

#188569

Purpose

green biosensor for cAMP in neurons

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1

Use

AAV

Tags

His tag

Expression

Mutation

Promoter

hSyn

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMIN-Ri

Plasmid

#197784

Purpose

Level 2 destination vector: pMIN-Ri backbone

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSL3005

Plasmid

#139977

Purpose

Suicide plasmid for integration of PlacUV5-cscB and lacI into Synechococcus sp. UTEX 2973 Neutral site III

Depositor

Himadri Pakrasi

Article

Lin et al Sci Rep. 2020 Jan 15;10(1):390. doi: 10.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE46

Plasmid

#90384

Purpose

NFYA - constitutive enhancer gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EGR1

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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NMD2

Plasmid

#216220

Purpose

Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_b-globin intron x2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

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XLone-Puro eGFP

Plasmid

#140027

Purpose

Tunable and temporal expression control of nuclear eGFP

Depositor

Xiaoping Bao

Insert

Enhanced Green Fluorescent Protein

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMK419 (GAL-OsTIR1(F74G))

Plasmid

#140656

Purpose

GAL-OsTIR1(F74G)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74G)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-DARPinOFF7

Plasmid

#182398

Purpose

Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-DARPinOFF7

Use

Tags

Vesicle Nucleating peptide 6 (VNp6)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.PAC

Plasmid

#57825

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
PAC

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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