-
Plasmid#80796PurposeExpression of immunoglobulin light chains in mammalian cells, human kappa constant regionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only
-
pCMV-mTagBFP2-ORP9-PH
Plasmid#214267PurposeMammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PHDepositorInsertmTagBFP2-ORP9(1-111) (Osbpl9 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
H1.M11.luc
Plasmid#193707PurposeExpressed a H1-core mutant by introducing multiple 7SK elements (octamer, PSE, spacer, and TATA box)DepositorInsertMutated H1-core promoter with multiple 7SK elements (octamer, PSE, spacer, and TATA box)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIG (w497-1)
Plasmid#26286DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH
Plasmid#185713PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
UseTags6xHis and MBPExpressionBacterialMutationChanged W403 and F753 to APromoterT7Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_PLCG1:1-1291
Plasmid#211089PurposeMammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.DepositorInsertPLCG1:M1-L1291 (PLCG1 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationisoform 2. I813T natural variant (VAR_011908)PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSExpressionMutationPromoterTRE, PGKAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_HA-BirA-ER
Plasmid#113897PurposeMammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligaseDepositorInsertE. coli biotin ligase
UseLentiviralTagsHAExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR FGFR1-CD3ε-FusionRed
Plasmid#188632PurposeHuman FGFR1 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensorDepositorInsertFGFR1 (FGFR1 Human, Synthetic)
UseLentiviralTagsCD3ε-FusionRedExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)
Plasmid#140005PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only