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Plasmid#60824Purposeto purify human ITPA from bacteriaDepositorInsertITPA (ITPA Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS315-48nt-telo-I-SceI
Plasmid#67623Purposetelomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeatsDepositorInsertsUseYeast cen/ars plasmidTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127316PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST
Plasmid#127318PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS315-0nt-telo-I-SceI
Plasmid#67624Purposetelomere cassette for telomere formation system containing 0 bp of S. pombe telomere repeatsDepositorInsertsUseYeast cen/ars plasmidTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1-IRES2-EGFP
Plasmid#170820Purposehuman GABAA receptors alpha1 subunit and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 4xSBR2 MutSBE
Plasmid#15707DepositorInsertp15 4xSBR2 (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationMutant SBE (Smad binding element)PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/GS-DJ1-E64D-V5
Plasmid#29344DepositorInsertDJ1 (PARK7 Human)
UseTagsHis6 and V5 epitopeExpressionMammalianMutationGlu64AspPromoterAvailable sinceApril 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
Flag-Fos S374A
Plasmid#118302PurposeExpression of mouse C-FosDepositorInsertC-Fos S374A (Fos Mouse)
UseTagsFlagExpressionMammalianMutationS374APromoterAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/GS-DJ1-D149A-V5
Plasmid#29345DepositorInsertDJ1 (PARK7 Human)
UseTagsHis6 and V5 epitopeExpressionMammalianMutationAsp149AlaPromoterAvailable sinceApril 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 4xSBR1 MutSBE
Plasmid#15718DepositorInsertp15 4xSBR1 (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationMutant SBE (Smad binding element)PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/GS-DJ1-E18Q-V5
Plasmid#29342DepositorInsertDJ1 (PARK7 Human)
UseTagsV5 epitopeExpressionMammalianMutationE18QPromoterAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/GS-DJ1-R98Q-V5
Plasmid#29340DepositorInsertDJ1 (PARK7 Human)
UseTagsHis6 and V5ExpressionMammalianMutationArg98GlnPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225649PurposetetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA, araBAD and LacO3O1 in tandem conformation
UseTagsmCherryExpressionBacterialMutationPromotertetA, araBAD and LacO3O1 promotersAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B2-ELP-HIS-ybbR
Plasmid#169134PurposeE. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B1-ELP-HIS-ybbR
Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSII-PGK-FLPo-IRES-Puro
Plasmid#205989PurposeExpresses Flp recombinase and puromycin resistanceDepositorInsertsPGK promoter
FLPo
IRES-PuroR cassette
UseTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1-Cdc45
Plasmid#208796PurposeXenopus laevis FLAG5-His10-TEV-Cdc45DepositorInsertCdc45 (cdc45.L Frog)
UseTagsFLAG and HisExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-WM
Plasmid#208798PurposeXenopus laevis His6-Myc-DONSON (W234L, M463T)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationW234L; M463TPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON-S437A
Plasmid#208803PurposeXenopus laevis His6-Myc-DONSON (S437A)DepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationS437APromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only