We narrowed to 4,870 results for: golden gate

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pVP081

Plasmid

#222033

Purpose

MultiGreen 2.0 DE level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP082

Plasmid

#222034

Purpose

MultiGreen 2.0 EF level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP083

Plasmid

#222035

Purpose

MultiGreen 2.0 FG level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGGPK-AG2

Plasmid

#222027

Purpose

Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBH111

Plasmid

#215778

Purpose

NBU2 backbone with anti-LacI(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

anti-LacI(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(O1)

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH101

Plasmid

#215772

Purpose

NBU2 backbone with LacI(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

BT1311 and P(O1)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH102

Plasmid

#215773

Purpose

NBU2 backbone with LacI(TAN) and P(tta)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(TAN)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(tta)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH103

Plasmid

#215774

Purpose

NBU2 backbone with LacI(KSL) and P(agg)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(KSL)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(agg)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH104

Plasmid

#215775

Purpose

NBU2 backbone with LacI(HQN) and P(ttg)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(HQN)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(ttg)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH105

Plasmid

#215776

Purpose

NBU2 backbone with LacI(GKR) and P(gac)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(GKR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(gac)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH106

Plasmid

#215777

Purpose

NBU2 backbone with RbsR(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

RbsR(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(O1)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBH116

Plasmid

#215779

Purpose

NBU2 backbone with anti-RbsR(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

anti-RbsR(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(O1)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH501

Plasmid

#215780

Purpose

NBU1 backbone with constitutive dCas9

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Insert

dCas9

Use

Synthetic Biology

Expression

Bacterial

Promoter

P1

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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GGDest1-Amp

Plasmid

#157649

Purpose

Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGGA008

Plasmid

#48817

Purpose

Provides the alcA promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

alcA promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

Plasmid

#228663

Purpose

Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika virus portion of NS2B protease coexpressed with NS3 protease

Tags

His-GST-TEV

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK1062

Plasmid

#236186

Purpose

BsaI compatible kanamycin resistance cassette

Depositor

Jeffrey Barrick

Article

VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

Insert

Kanamycin resistance cassette

Expression

Bacterial

Promoter

CP25

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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cwby0082

Plasmid

#247432

Purpose

GGA cloning AGGA-H6-GS-promoter-ccdb-TTCC-SNAC-6xhis

Depositor

David Baker

Article

Qian et al bioRxiv 2025.08.05.668824; doi: https://

Insert

ccdb

Expression

Bacterial

Promoter

T7

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMB1o1_Red

Plasmid

#243862

Purpose

GoldenBraid2.0 expression vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

mScarlet-I

Expression

Bacterial

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pJet1.2 sfGFP

Plasmid

#117123

Purpose

C-terminal GFP tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Superfolder GFP

Use

Golden gate donor vector for gene targeting in ba…

Tags

sfGFP

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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