We narrowed to 4,633 results for: golden gate

Showing: 1861 - 1880 of 4633 results

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pBH104

Plasmid

#215775

Purpose

NBU2 backbone with LacI(HQN) and P(ttg)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(HQN)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(ttg)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH105

Plasmid

#215776

Purpose

NBU2 backbone with LacI(GKR) and P(gac)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(GKR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(gac)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH106

Plasmid

#215777

Purpose

NBU2 backbone with RbsR(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

RbsR(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(O1)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH116

Plasmid

#215779

Purpose

NBU2 backbone with anti-RbsR(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

anti-RbsR(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(O1)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH501

Plasmid

#215780

Purpose

NBU1 backbone with constitutive dCas9

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Insert

dCas9

Use

Synthetic Biology

Expression

Bacterial

Promoter

P1

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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GGDest1-Amp

Plasmid

#157649

Purpose

Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLSL.R.Ly

Plasmid

#227697

Purpose

Reengineered geminiviral replicon vector

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

Plasmid

#228663

Purpose

Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika virus portion of NS2B protease coexpressed with NS3 protease

Tags

His-GST-TEV

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK1062

Plasmid

#236186

Purpose

BsaI compatible kanamycin resistance cassette

Depositor

Jeffrey Barrick

Article

VanDieren et al MicroPubl Biol. 2025 Aug 2;2025:10.17912

Insert

Kanamycin resistance cassette

Expression

Bacterial

Promoter

CP25

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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cwby0082

Plasmid

#247432

Purpose

GGA cloning AGGA-H6-GS-promoter-ccdb-TTCC-SNAC-6xhis

Depositor

David Baker

Article

Qian et al bioRxiv 2025.08.05.668824; doi: https://

Insert

ccdb

Expression

Bacterial

Promoter

T7

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMB1o1_Red

Plasmid

#243862

Purpose

GoldenBraid2.0 expression vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

mScarlet-I

Expression

Bacterial

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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DVC_p15A_AF

Plasmid

#217588

Purpose

Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJet1.2 sfGFP

Plasmid

#117123

Purpose

C-terminal GFP tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Superfolder GFP

Use

Golden gate donor vector for gene targeting in ba…

Tags

sfGFP

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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cwby0001

Plasmid

#232210

Purpose

GGA cloning M-AGGA-promoter-ccdb-TTCC-SNAC-HIS

Depositor

David Baker

Article

Qian et al bioRxiv 2025.08.05.668824; doi: https://

Insert

ccdb

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAttBF3Hyg

Plasmid

#244885

Purpose

Hyg resistant attB phiC31 recombination-mediation insertion (RMI) vector for C. elegans

Depositor

Michael Nonet

Article

Beck et al MicroPubl Biol. 2025 Sep 2;2025:10.17912

Type

Empty backbone

Use

Phic31 recombinase mediated insertion vector

Expression

Worm

Promoter

none

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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DVA_p15A_AH

Plasmid

#217602

Purpose

Destination vector with ampicillin resistance and p15A origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228653

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

MVEV portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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DVA_pBR322_AG

Plasmid

#217607

Purpose

Destination vector with ampicillin resistance and pBR322 origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DVC_pBR322_FG

Plasmid

#217600

Purpose

Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pICSL60004

Plasmid

#117519

Purpose

rbcS-E9 terminator Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GCTT:E9t:CGCT:BsaI

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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