Addgene

We narrowed to 3,890 results for: chm

Showing: 1861 - 1880 of 3890 results

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pMaCTag-P30

Plasmid

#120041

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

VSV-G

Use

Pcr template

Tags

VSV-G

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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GTU-r

Plasmid

#81096

Purpose

Gene Tagging vector URA/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCherry

Use

Cre/Lox

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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U6d

Plasmid

#117224

Purpose

Expresses gRNA in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

Insert

HR U6d promoter-gRNA

Use

Synthetic Biology

Tags

Expression

Insect

Mutation

Promoter

U6

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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U6a

Plasmid

#117221

Purpose

Expresses gRNA in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

Insert

HR U6a promoter-gRNA

Use

Synthetic Biology

Tags

Expression

Insect

Mutation

Promoter

U6

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-25

Plasmid

#120004

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG301

Plasmid

#105358

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor DD45/EC1.2 (At2g21740;1000bp)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG658

Plasmid

#105409

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

3xHA tag fused to Luciferase CDS

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG298

Plasmid

#105361

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor ICU2 (At5g67100;600bp)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-19

Plasmid

#124787

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Expression

Mutation

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG642

Plasmid

#105406

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

3xHA tag fused to tagRFP-T CDS

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2-FW/AA

Plasmid

#12293

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y, F774A and W775A; signal peptide (aa1…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCK015

Plasmid

#105347

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Tags

Expression

Mutation

BsaI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG644

Plasmid

#105352

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Gal4UAS & 35S min promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P03

Plasmid

#120014

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCK025

Plasmid

#105415

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Hormone-binding domain and regulatory sequences (estrogen receptor)

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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GTL-r

Plasmid

#81195

Purpose

Gene Tagging vector LEU/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCherry

Use

Cre/Lox

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pJW4.17

Plasmid

#62872

Purpose

Bacterial expression of MBP-MBK-2 K196R

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2 K196R

Use

Tags

MBP

Expression

Bacterial

Mutation

K196R

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only
pEC2861 (pSpy1C-PgyrA_ffluc)

Plasmid

#218511

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Mutation

deletion of Plac_mrfp cassette

Promoter

PgyrA

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3105 (pSpy1C-PxylS2_ffluc)

Plasmid

#218512

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Mutation

deletion of Plac_mrfp cassette

Promoter

PxylS2

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro

Plasmid

#211622

Purpose

Rhno1 S141A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Use

Tags

myc-flag

Expression

Mammalian

Mutation

S141A

Promoter

CMV

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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