Addgene

Showing: 1861 - 1880 of 11035 results

Results per page:

Sort results by:

CCR2-DuET

Plasmid

#213198

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCR2 (CCR2 Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pv6_MECP2_MBD

Plasmid

#179407

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-10-3xFLAG-LGP2-K651E

Plasmid

#58685

Purpose

Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells

Depositor

Curt Horvath

Article

Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

Insert

LGP2-K651E (DHX58 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

mutation to CTD, K651E

Promoter

CMV

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only
pHIV-EGFP.Flag-EZH2-mt2*

Plasmid

#220246

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2* (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

PRKKKR494-499NAAIRS

Promoter

EF-1α

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.EZH2-mt2

Plasmid

#220240

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Use

Tags

6x His, MBP

Expression

Insect

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

Polyhedrin

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_1-590 POLI

Plasmid

#131233

Purpose

Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_1-420 POLI

Plasmid

#131234

Purpose

Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK603

Plasmid

#8831

Depositor

David Waugh

Article

Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

Insert

TEV protease, S219D mutant

Use

Tags

Expression

Bacterial

Mutation

S219D mutant

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
UAS-rhophilin

Plasmid

#41974

Depositor

Norbert Perrimon

Article

Hacker et al Genes Dev. 1998 Jan 15;12(2):274-84.

Insert

rhophilin

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Feb. 21, 2013

Availability

Academic Institutions and Nonprofits only
pGolt1-mCherry

Plasmid

#73361

Purpose

In vivo protein protein interaction studies/ expression in mammalian cells

Depositor

Michael Kreutz

Insert

TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV immediate early promoter

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGolt2-mCherry

Plasmid

#73358

Purpose

In vivo protein protein interaction studies/ expression in mammalian cells

Depositor

Michael Kreutz

Insert

TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV immediate early promoter

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
IDG_KCNH6_OE_1

Plasmid

#161669

Purpose

Overexpresses KCNH6

Depositor

Mike McManus

Insert

KCNH6 (KCNH6 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/FGFR2myc

Plasmid

#33248

Depositor

Alka Mansukhani, Claudio Basilico

Article

Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

Insert

FGFR2 (Fgfr2 Mouse)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

KpnI site introduced at stop codon of FGFR2 to be…

Promoter

CMV

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
CMV-218_HNF4A2

Plasmid

#31090

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 . 2():210.

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 2 of HNF4A, deletion of CMV promo…

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-68_HNF4A2

Plasmid

#31092

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 . 2():210.

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 2 of HNF4A, deletion of CMV promo…

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-138_HNF4A2

Plasmid

#31091

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 . 2():210.

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

splice variant 2 of HNF4A, deletion of CMV promo…

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pSth_Cas6

Plasmid

#178779

Purpose

Expression of S. thermophilus type I-E cas6

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cas6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSth_Cas8

Plasmid

#178776

Purpose

Expression of S. thermophilus type I-E cas8

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cas8

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSth_Cse2

Plasmid

#178777

Purpose

Expression of S. thermophilus type I-E cse2

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cse2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSth_Cas5

Plasmid

#178778

Purpose

Expression of S. thermophilus type I-E cas5

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cas5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge