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Plasmid#176736PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Kluyveromyces lactis (barrier part).DepositorArticleInsertbar1Kl
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK033
Plasmid#176737PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Kazachstania naganishii (barrier part).DepositorArticleInsertbar1Kn
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK017
Plasmid#176721PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Tetrapisispora phaffii (sender part).DepositorArticleInsertmfalpha1Tp
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK012
Plasmid#176716PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania naganishii (sender part).DepositorArticleInsertmfalpha1Kn
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK018
Plasmid#176722PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Vanderwaltozyma polyspora (sender part).DepositorArticleInsertmfalpha1Vp
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK015
Plasmid#176719PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Lachancea thermotolerans (sender part).DepositorArticleInsertmfalpha1Lt
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK014
Plasmid#176718PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Lachancea mirantina (sender part).DepositorArticleInsertmfalpha1Lm
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK011
Plasmid#176715PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kluyveromyces lactis (sender part).DepositorArticleInsertmfalpha1Kl
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAKW.114A-PKA
Plasmid#154051PurposeExpresses PKA in E. coliDepositorInsertProtein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
UseTagsExpressionBacterialMutationPromoterpCinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPer-Red
Plasmid#48249PurposeGenetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHYPER-RED
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-A3-HA-ubiquitin
Plasmid#220145PurposeExpresses A3-Ub for E3 substrate identification by E-STUBDepositorInsertUbiquitin C (UBC Human)
UseTagsA3-HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-AsCas12f-sgRNA-v2
Plasmid#204639PurposeExpression of trancated AsCas12f sgRNA in mammalian cellsDepositorInserttrancated AsCas12f sgRNA (sgRNA-v2)
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATeam1.03-nD/nA/pcDNA3
Plasmid#51958Purposemammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Orange_CaMBI_110
Plasmid#124094Purposeorange calcium-modulated bioluminescent indicator, 110nMDepositorInsertOrange CaMBI 110
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-BRIC
Plasmid#172341PurposeAAV transfer plasmid for BRIC (Bioluminescent Red Indicator for Calcium)DepositorInsertBRIC
UseAAVTagsExpressionMutationPromoterhSyn promoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-TALO8
Plasmid#83409PurposeTRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeastDepositorInsertTRP-ARS-8xLacO
UseTagsExpressionYeastMutationPlease see depositor comments belowPromoterTRP1Available sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Orange_CaMBI_300
Plasmid#124095Purposeorange calcium-modulated bioluminescent indicator, 300nMDepositorInsertOrange CaMBI 300
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal:mCherry-uTEV3
Plasmid#135453PurposeYeast expression of uTEV3 fused to mCherryDepositorInsertmCherry-uTEv3
UseTagsmCherryExpressionYeastMutationS219V mutation improves stability , I138T/S153N/T…PromoterGalAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ateam1.03-nD/nA/pRSETB
Plasmid#51960Purposebacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only