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Showing: 1861 - 1880 of 73793 results

  1. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

    Plasmid
    #172597
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-K627R (PDGFRA Synthetic, Human)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-K627R
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172597-plasmid-map-sequence-id-338908

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  2. NODAL-MYC-DYK-N72A-N199A

    Plasmid
    #115252
    Purpose
    For mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    N72A, N199A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115252-plasmid-map-sequence-id-222234

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  10. pCEP4_HLA-cmyc-GRM3 C127A T174A-VC

    Plasmid
    #140457
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A mutant fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, Threonine 174 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140457-plasmid-map-sequence-id-292299

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  11. pCEP4_HA-Halo-cmyc-GRM3 C127A

    Plasmid
    #140458
    Purpose
    Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Halo, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, Threonine 174 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140458-plasmid-map-sequence-id-292298

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  14. pcDNA3.1-kappa-myc-dK-2xG4S-TMst

    Plasmid
    #145766
    Purpose
    Expresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.
    Depositor
    Marcel Bruchez
    Article
    Carpenter et al ACS Chem Biol. 2020 Aug 17. doi: 10.1021
    Insert
    kappa-myc-dK-2xG4S-TMst
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The dK FAP is dL5 with mutations at E50K, L89S, a…
    Promoter
    CMV
    Available since
    Jan. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    145766-plasmid-map-sequence-id-353271

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  16. pIRESneo2 mycBioID-Tollip M240A,F241A

    Plasmid
    #178055
    Purpose
    myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
    Depositor
    David Tumbarello
    Article
    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:
    Insert
    Toll-Interacting Protein (TOLLIP Human)
    Use
    Tags
    mycBioID
    Expression
    Mammalian
    Mutation
    Changed Methionine 240 to Alanine and changed Phe…
    Promoter
    CMV
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    178055-plasmid-map-sequence-id-350118

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  19. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

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Showing: 1861 - 1880 of 73793 results