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mEos2-Alpha-Actinin-19
Plasmid#57346PurposeLocalization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pN1-mCherry-NKD2
Plasmid#176641PurposeExpresses NKD2-mCherry fusion protein in mammalian cellsDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Alpha-Actinin-C-14
Plasmid#57347PurposeLocalization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac-His-Flag-Tipin-L195A
Plasmid#23120DepositorInsertTipin (TIPIN Human)
UseTagsFlag- and HisExpressionInsectMutationChanged Leucine 195 to AlaninePromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-Tipin-L195A
Plasmid#23121DepositorInsertTipin (TIPIN Human)
UseTagsHisExpressionInsectMutationChanged Leucine 195 to AlaninePromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBK1289-AAV-dCjCas9
Plasmid#223138PurposeAAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1290-AAV-dSaCas9
Plasmid#223139PurposeAAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Combined γB2FL-FRET probe
Plasmid#209094PurposemTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Combined γB2ΔICD-FRET probe
Plasmid#209093PurposemTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP12-talinC11-ABS3mut-mEmerald
Plasmid#202383PurposeFKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmeraldDepositorInsertFKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmerald
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2S31A
Plasmid#174332Purposeencodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationchanged Serine 31 to AlaninePromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-FokI-dCas9-EGFP-L2
Plasmid#141047PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-FokI-dCas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-Flag-Tipin-L195A
Plasmid#23118DepositorInsertTipin (TIPIN Human)
UseTagsFlagExpressionMammalianMutationChanged Leucine 195 to AlaninePromoterAvailable sinceFeb. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
PLJR965
Plasmid#115163PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosisDepositorInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLJR962
Plasmid#115162PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. smegmatisDepositorInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Actin-7
Plasmid#57339PurposeLocalization: Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertActin (ACTB Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE1059
Plasmid#186628PurposeExpression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.DepositorInsertsscbA
scbB
scbC
UseSynthetic BiologyTags6xHis and 6xHis-thrombinExpressionBacterialMutationPromoterSynthetic promoter B10, Synthetic promoter B19, a…Available sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Fla
Plasmid#84866PurposeBaculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Fla
UseBaculovirusTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only
