-
Plasmid#31213DepositorInsertMTHFD2 (MTHFD2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLC-EGFP-RIP3
Plasmid#75165PurposeLentiCRISPR-EGFP with sgRNA targeting human RIP3DepositorInsertRIP3 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8_Flag2-Blast
Plasmid#175038PurposeMammalian expression of full length mMPP8 with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationPromoterCAGAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLC-mCherry-MLKL
Plasmid#75167PurposeLentiCRISPR-mCherry with sgRNA targeting human MLKLDepositorInsertMLKL sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 UBR5 ∆HECT
Plasmid#81065PurposeGateway entry vector encoding full length UBR5 C2768A (∆HECT)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
UseCloningTagsExpressionMutationC2768A (bp t8302g and g8303c)PromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-Flag-mPKM2(C358S)
Plasmid#42513DepositorInsertPyruvate Kinase M2 Cys358->Ser
UseRetroviralTagsFlagExpressionMutationChanged Cysteine 358 to SerinePromoterCMVAvailable sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-act1p
Plasmid#120233PurposeCarries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing act1p promoter and apramycin resistance gene
UseSynthetic BiologyTagsExpressionMutationaac(3)IV (apramycin resistance gene)PromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127315PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST
Plasmid#127312PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDL024_Tub-rb_IgG_LC
Plasmid#193621Purposeα-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.DepositorInsertAnti-Tubulin scFv light variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-His-hPKM2
Plasmid#42515DepositorInsertPyruvate Kinase M2 (PKM Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-H2B-6
Plasmid#55056PurposeLocalization: Nucleus/Histones, Excitation: 587, Emission: 610DepositorInsertH2B (H2BC11 Human)
UseTagsmCherryExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLC-EGFP-RIP1
Plasmid#75163PurposeLentiCRISPR-EGFP with sgRNA targeting human RIPk1DepositorInsertRIPK1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-UCHL5
Plasmid#31219DepositorInsertUCHL5 (UCHL5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP EGFP-ER [TM(SAC1)]
Plasmid#186475PurposeExpression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cellsDepositorInsertSAC1 (SACM1L Human)
UseRetroviralTagsEGFPExpressionMammalianMutationC-terminal transmembrane region of the protein: 5…PromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-NDC80
Plasmid#31215DepositorInsertNDC80 (NDC80 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP ArgSen delta Rad23b UbL
Plasmid#184703PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbLDepositorInsertGFP ArgSen delta Rad23b UbL
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGW1-Myc-DJ1-L166P/K130R
Plasmid#29349DepositorInsertDJ1 (PARK7 Human)
UseTagsMycExpressionMammalianMutationLeu166Pro and Lys130ArgPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/GS-DJ1-L166P-V5
Plasmid#29343DepositorInsertDJ1 (PARK7 Human)
UseTagsHis6 and V5 epitopeExpressionMammalianMutationL166PPromoterAvailable sinceMarch 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_PTC160-xrRNA-4H
Plasmid#108369PurposeExpresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPTC160PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only