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Plasmid#106999PurposeTo express 6His-fSNAP protein in E.coliDepositorInsertfSNAP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-His-hCE
Plasmid#112710PurposeExpresses N-terminally His-tagged human CE/RNGTT in bacterial cellsDepositorInsertRNGTT (RNGTT Human)
UseTags6xHis and Stop codons placed after RNGTT sequence…ExpressionBacterialMutationR594HPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12c1
Plasmid#120872PurposeExpresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.DepositorInsertCas12c1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MinE-His
Plasmid#133623PurposeExpression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.DepositorInsertMinE-His (minE E. coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-LanCL1
Plasmid#154189PurposeTo express LanCL1 in bacterial cellsDepositorInsertLanCL1
UseTagsHexahistidineExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-8
Plasmid#172772PurposeBacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-Luc
Plasmid#135627PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)DepositorInsertNAD-Luc sensor
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2m2-SNAP
Plasmid#208773PurposeTo express C2m2-SNAP in bacteriaDepositorInsertC2m2-SNAP (Mfge8 Mouse)
UseTagsSNAP-tagExpressionBacterialMutationMutated 24 and 45 lysine to asparagine in C2 doma…PromoterT7 promoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2m2-mKate
Plasmid#208765PurposeTo express C2m2-mKate in bacteriaDepositorInsertC2m2-mKate (Mfge8 Mouse)
UseTagsmKateExpressionBacterialMutationMutated 24 and 45 lysine to asparagine in C2 doma…PromoterT7 promoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b-eGO-Matryoshka-LE-LP
Plasmid#100017PurposeBacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpEGFP-LSSmOrange
UseTags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET26-Ufd1-His
Plasmid#21266DepositorInsertUfd1 (Ufd1l Mouse)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-TBP6.9F34MF37MF77M
Plasmid#168268PurposeExpresses human TBP6.9 with the F34M/F37M/F77M triple mutantDepositorInsertTBP6.9
UseTags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F34M, F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 P186C
Plasmid#66173PurposeExpresses the P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationP186CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-FB-E25TAG-F6C
Plasmid#176546PurposeExpresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
UseTagsHis tagExpressionBacterialMutationchange phenylalanine 6 to cysteine; change glutam…PromoterT5Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::MBP-NL3F10H
Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-17
Plasmid#172747PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-17
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-dH3-dH4
Plasmid#105502PurposeCo-expression of Drosophila histones H3 and H4DepositorInsertdH3-dH4
UseTagsExpressionMutationPromoterAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-HIS-Sangamo
Plasmid#40786Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsN Terminal 6xHISExpressionBacterialMutation+63 C Terminus, Codon Optimized FokI EndonucleasePromoterT7Available sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-myc
Plasmid#109422PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and mycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET23-CBD-(-PP)-EGFP
Plasmid#12597DepositorInsertCdc42 Biosensor (WAS Human)
UseTags6xHis and EGFPExpressionBacterialMutationF271CPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only