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We narrowed to 20,410 results for: crispr

Showing: 1841 - 1860 of 20410 results

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peSpCas9(1.1)-2×sgRNA (empty, donor)

Plasmid

#80768

Purpose

All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAc-y1sgRNA-Cas9

Plasmid

#49331

Purpose

Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA
y1 sgRNA (y Fly)

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Availability

Academic Institutions and Nonprofits only

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pAC2-dual-dCas9VP48-sgExpression

Plasmid

#48236

Purpose

Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

Availability

Academic Institutions and Nonprofits only

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pAC5-dual-dCas9VP48-sgTetO

Plasmid

#48237

Purpose

Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48 and sgTetO

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

Availability

Academic Institutions and Nonprofits only

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pKSE401

Plasmid

#62202

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

35S and AtU6-26p

Availability

Academic Institutions and Nonprofits only

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BPK764

Plasmid

#65767

Purpose

Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Bacterial

Mutation

Promoter

T7 (x2)

Availability

Academic Institutions and Nonprofits only

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MSP1673

Plasmid

#65769

Purpose

Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA

Use

CRISPR

Tags

NLS

Expression

Bacterial

Mutation

Promoter

T7 (x2)

Availability

Academic Institutions and Nonprofits only

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BPK2101

Plasmid

#65770

Purpose

Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized SaCas9, and SaCas9 gRNA

Use

CRISPR

Tags

NLS-3xFlag

Expression

Bacterial

Mutation

Promoter

T7 (x2)

Availability

Academic Institutions and Nonprofits only

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pML104

Plasmid

#67638

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
single guide RNA expression cassette

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

pSNR52 and pTDH3

Availability

Academic Institutions and Nonprofits only

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pML107

Plasmid

#67639

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
single guide RNA expression cassette

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

pSNR52 and pTDH3 (aka GAP promoter)

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

Plasmid

#71236

Purpose

Express sgRNA and dCas9-KRAB from lentiviral vector

Depositor

Charles Gersbach

Article

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A Puro
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mutation

D10A and H840A

Promoter

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-Puro

Plasmid

#86708

Purpose

Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

Depositor

Christoph Bock

Article

Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

Insert

EF1a-Puro-WPRE-hU6-gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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pSC51

Plasmid

#104825

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.12g075700, Glyma.11g145900

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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BB3cN_pGAP_23*_pLAT1_Cas9

Plasmid

#104906

Purpose

hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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BB3cK_pGAP_23*_pLAT1_Cas9

Plasmid

#104908

Purpose

hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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BB3cK_pGAP_23*_pPFK300_Cas9

Plasmid

#104910

Purpose

hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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BB3cH_pGAP_23*_pPFK300_Cas9

Plasmid

#104912

Purpose

hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

Plasmid

#102887

Purpose

PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-P300-T2A-GFP-IRES-Neo

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

Plasmid

#102891

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-P300-T2A-GFP-IRES-Neo

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Availability

Academic Institutions and Nonprofits only

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