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Cry2L348F(1-531)-mCh-BAXS184E

Plasmid

#117239

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531.L348F-mCh-BAXS184E

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab32a

Plasmid

#109621

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab32a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab32a (rab32a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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8xCTS1_BpiI-cloning-site_SV40polyA

Plasmid

#124547

Purpose

Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-eGFP-NES-NFATC2IP

Plasmid

#221446

Purpose

Mammalian expression vector of eGFP tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Tags

Nuclear Export Signal (NES) and eGFP

Expression

Mammalian

Mutation

NES (NLVDLQKKLEELELDEQQ) inserted, 1st Methionine…

Promoter

CMV

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-3xFLAG-NFATC2IP ∆101-140

Plasmid

#221212

Purpose

Mammalian expression vector of triple Flag tagged NFATC2IP with amino acids 101-140 deleted

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

1st Methionine and amino acids 101-140 deleted

Promoter

CMV

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-3XFLAG-NFATC2IP SLD2

Plasmid

#221388

Purpose

Mammalian expression vector of triple Flag tagged NFATC2IP ORF SUMO-like domain 2 (SLD2, aa 345-418)

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Amino acids 1-344 of NFATC2IP deleted

Promoter

CMV

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-3XFLAG-SLD1+SLD2

Plasmid

#221389

Purpose

Mammalian expression vector of triple Flag tagged NFATC2IP ORF SUMO-like domain 1 and SUMO-like domain 2 (SLD1+SLD2, aa 251-419)

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Amino acids 1-250 of NFATC2IP deleted

Promoter

CMV

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-eGFP-NFATC2IP ∆SLD1

Plasmid

#221434

Purpose

Mammalian expression vector of eGFP tagged NFATC2IP ORF with SUMO-like domain 1 (SLD1, aa 256-344) deleted

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

SUMO-like domain 1 (SLD1, aa 256-344) deleted, 1s…

Promoter

CMV

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT/T0-eGFP-NFAT2CIP ∆101-140

Plasmid

#221437

Purpose

Mammalian expression vector of eGFP tagged NFATC2IP with amino acids 101-140 deleted

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

1st Methionine and amino acids 101-140 deleted

Promoter

CMV

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT/T0-eGFP-SLD1+SLD2

Plasmid

#221443

Purpose

Mammalian expression vector of egFP tagged NFATC2IP ORF SUMO-like domain 1 and SUMO-like domain 2 (SLD1+SLD2, aa 251-419)

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Amino acids 1-250 of NFATC2IP deleted

Promoter

CMV

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT/T0-3XFLAG-NES-NFATC2IP

Plasmid

#221444

Purpose

Mammalian expression vector of triple Flag tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Tags

3XFlag and Nuclear Export Signal (NES)

Expression

Mammalian

Mutation

NES (NLVDLQKKLEELELDEQQ)inserted, 1st Methionine …

Promoter

CMV

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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RC(14-3-3ε)-LgBit

Plasmid

#208926

Purpose

Recognition domain for in vitro RSK-activity sensor

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

14-3-3ε (YWHAQ Human)

Use

Tags

HISx6, LgBit, and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSLIK BirA* 3xFLAG-NUP37 zeo

Plasmid

#192333

Purpose

Lentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-NUP37

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*-3xFLAG-NUP37 (NUP37 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT BirA*-3xFLAG-NUP37

Plasmid

#192311

Purpose

Gateway entry vector for an inducible 3xFLAG-BirA* 3xFLAG-NUP37

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*-3xFLAG-NUP37 (NUP37 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAM1160c2

Plasmid

#159724

Purpose

Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Use

Tags

Gal4DBD and MPGIL-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1674

Plasmid

#159726

Purpose

Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Use

Tags

Gal4DBD and MPTLV-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAM1479a3

Plasmid

#159725

Purpose

Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

mDHFR (Dhfr Mouse)

Use

Tags

Gal4DBD and MYITV-GGGGGG

Expression

Yeast

Mutation

remove start and stop codon of Dhfr

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMT-Map7-1783-end

Plasmid

#46071

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-1783-end (Map7 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEMT-Map7-856-1351

Plasmid

#46069

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-856-1351 (Map7 Mouse)

Use

Unspecified

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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KHBD00396

Plasmid

#34507

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8961 (tef Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only