Addgene

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pDSM-cd-Pfba1p-ACO-Tgpm1

Plasmid

#127731

Purpose

Yeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pfba1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pykt6-ACO-Tprm9

Plasmid

#127732

Purpose

Yeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pykt6

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Ptef1-ACO-Tyol036w

Plasmid

#127733

Purpose

Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ptef1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ptef1-CSC-Tpdc1

Plasmid

#127739

Purpose

Yeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pfba1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Pzuo1-CSC-Tvma16

Plasmid

#127740

Purpose

Yeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pzuo1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ppfy1-CSC-Taip1

Plasmid

#127741

Purpose

Yeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ppfy1

Available since

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Marvin Wickens, Hugo Cesar Medina-Munoz

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

PSEC63

Available since

Availability

Academic Institutions and Nonprofits only

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FRP272

Plasmid

#159305

Purpose

Yeast tagging vector to create gene fusion with sfGFP under KanMX selection

Depositor

Joerg Stelling

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415GPD-EGFP-ccdB

Plasmid

#14314

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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YIplac211-Vph1-HaloTag

Plasmid

#140149

Purpose

Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-HaloTag

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

VPH1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGBKCg

Plasmid

#20162

Depositor

Peter Uetz

Article

Stellberger et al Proteome Sci. 2010 . 8():8.

Type

Empty backbone

Use

Tags

Gal4 DBD

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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pAG306GPD-ccdB-DsRed

Plasmid

#14356

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

DsRed

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pJH124

Plasmid

#102951

Purpose

Expresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoter

Depositor

Peter Espenshade

Article

Hwang et al Biochem J. 2016 Jun 7. pii: BCJ20160106.

Insert

APEX2-NES

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pGADCg

Plasmid

#20161

Depositor

Peter Uetz

Article

Stellberger et al Proteome Sci. 2010 . 8():8.

Type

Empty backbone

Use

Tags

Gal4 AD and SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG426GPD-ccdB-HA

Plasmid

#14252

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-ccdB

Plasmid

#14147

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway destination

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG426GAL-ccdB-EGFP

Plasmid

#14203

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pKT0210

Plasmid

#8724

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP A206R

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pFS255

Plasmid

#12382

Depositor

Nick Rhind

Article

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

thymidine kinase

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG425GAL-ccdB

Plasmid

#14153

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway destination

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge