-
Plasmid#157672PurposeExpression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertCohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
UseTagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialMutationPromoterT7Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
csd5_41-192/pET28b(+)
Plasmid#83296Purposecsd5, construct: 41-192, pET28b(+) N-tagDepositorInserthp1250
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-40PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd4_22-438/pET28b(+)
Plasmid#83295Purposecsd4, construct: 22-438, pET28b(+) N-tagDepositorInserthp1075
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-21PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BeGFP
Plasmid#21210DepositorInsertPhosphoglycerate kinase promoter (PGK1 Human)
UseLentiviralTagsH2B-eGFPExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDM002-sgRNA-lenti-ZeoR
Plasmid#200060PurposeLentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sitesDepositorInsertBleomycin resistance, mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BmCherry
Plasmid#21217DepositorInsertPhosphoglycerate kinase promoter (PGK1 Human)
UseLentiviralTagsH2B-mCherryExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST
Plasmid#127314PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCTRE-CD4
Plasmid#114010PurposeDox-inducible Cas9 expression with a CD4 selection markerDepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-2x-Rhotekin-GBD
Plasmid#214142PurposeRho activity sensorDepositorInsert3xRhotekin-GBD (Rtkn Mouse)
UseTagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
LZRS-HOTAIR
Plasmid#26110DepositorInsertHOTAIR (HOTAIR Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDY118A
Plasmid#182958PurposepSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.DepositorInsertsCas9
Gam, Beta, Exo
I-scel
UseTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSaPIPLC His
Plasmid#173810PurposeExpression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertStaphylococcus aureus plc
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH2R-mCherry-N1
Plasmid#84326PurposeHistamine-2 receptor tagged with mCherryDepositorInsertH2R (HRH2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH3R-mCherry-N1
Plasmid#84327PurposeHistamine-3 receptor tagged with mCherryDepositorInsertH3R-mCherry (HRH3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON
Plasmid#208797PurposeXenopus laevis His6-Myc-DONSON wild typeDepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-NCAPH
Plasmid#31214DepositorInsertNCAPH (NCAPH Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDRM98 mKate2 Blast
Plasmid#174720PurposeExpresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.DepositorInsertsmKate2
BSD
UseLentiviralTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianMutationPromoterMND and MND (off mKate-P2A)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 UBR5 ∆UBR
Plasmid#81063PurposeGateway entry vector encoding full length UBR5 W1235L (∆UBR)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
UseCloningTagsExpressionMutationW1235L (bp g3704t)PromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDL023_Tub-rb_IgG_HC
Plasmid#193620Purposeα-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.DepositorInsertAnti-Tubulin scFv heavy variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits