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Plasmid#216362PurposexhNup98-Nb2i Nanobody productionDepositorInsertxhNup98-Nb2i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC130
Plasmid#216364PurposexhNup98-Nb3i Nanobody productionDepositorInsertxhNup98-Nb3i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDG04477
Plasmid#216352PurposexhNup35-Nb1t Nanobody productionDepositorInsertxhNup35-Nb1t
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S1.46G>U
Plasmid#204160PurposepRegev_S1 with single mutation that disrupts secondary structure.DepositorInsertS1 exon with 46G>U substitution in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation46G>U substitutionPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S2.39U>G
Plasmid#204163PurposepRegev_S2 with single mutation that disrupts secondary structure.DepositorInsertExon S2 with 39U>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.39U>G.51A>C
Plasmid#204173PurposepRegev_S4 with double mutations that restore secondary structure.DepositorInsertExon S4 with 39U>G and 51A>C mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G and 51A>C mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S1.34C>A_46G>U
Plasmid#204161PurposepRegev_S1 with double mutations that restore secondary structure.DepositorInsertS1 exon with 34C>A and 46G>U substitutions in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation34C>A and 46G>U mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.51A>C
Plasmid#204172PurposepRegev_S4 with single mutation that disrupts secondary structure.DepositorInsertExon S4 with 51A>C mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation51A>C mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.39U>G
Plasmid#204171PurposepRegev_S4 with single mutation that disrupts secondary structure.DepositorInsertExon S4 with 39U>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S2.39U>G.51A>C
Plasmid#204165PurposepRegev_S2 with double mutations that restore secondary structure.DepositorInsertExon S2 with 39U>G and 51A>C mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation39U>G and 51A>C mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S5.49C>A
Plasmid#204175PurposepRegev_S5 with single mutation that disrupts secondary structure.DepositorInsertExon S5 with 49C>A mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation49C>A mutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S5.49C>A.59G>U
Plasmid#204177PurposepRegev_S5 with double mutations that restore secondary structure.DepositorInsertExon S5 with 49C>A and 59G>U mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation49C>A and 59G>U mutationsPromoterTruncated CAGAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO5
Plasmid#193749PurposeExpresses tdTomato under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selectionDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterPIAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO3
Plasmid#193748PurposeExpresses E2-Crimson under the control of PAAH synthetic promoter regulated by AHL and aTc, p15A origin of replication, Chloramphenicol selectionDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPAAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO6
Plasmid#193747PurposeExpresses E2-Crimson under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selection,DepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPIAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR
Plasmid#193746PurposeExpresses LuxR under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertLuxR
UseTagsExpressionBacterialMutationPromoterBBa_J23102Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-matDYK
Plasmid#115260PurposeFor mammalian expression of a human NODAL splice varaint open reading frame with an internal DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-matNODALvar-HIS
Plasmid#115265PurposeFor bacterial recombinant protein production of the mature peptide of a human NODAL splice variantDepositorInsertNODAL variant mature peptide (NODAL Human)
UseTags6HisExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-MYC-DYK
Plasmid#115245PurposeFor mammalian expression of a human NODAL splice variant open reading frame with a C-terminal MYC-DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsMYC-DYKExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR
Plasmid#178905Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationPromoterSV40 promoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only