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Plasmid#186911PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)1-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-1xL
Plasmid#186907PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ammB3
Plasmid#167815PurposeExpresses His6-tagged AmmB3 in E. coliDepositorInsertAmmB3
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ammC1
Plasmid#167816PurposeExpresses His6-tagged AmmC1 in E. coliDepositorInsertAmmC1
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGPN
Plasmid#187482PurposeExpression vector - GFPDepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK C-term
Plasmid#186147PurposeFor expresseion of recombinant FAK c-terminal domain in E. coliDepositorInsertPTK2 (PTK2 Mouse)
UseTagsHis6-sumoExpressionBacterialMutationPromoterT7Available sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLucia
Plasmid#135287PurposeGBPart - Lucia Luciferase CDSDepositorInsertLucia Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRoLuc
Plasmid#135284PurposeGBPart - RoLuc Luciferase CDSDepositorInsertR. ohbai Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMetLuc
Plasmid#135286PurposeGBPart - MetLuc Luciferase CDSDepositorInsertMetridia Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-NHX1-iGFP
Plasmid#177108PurposeExpresses Nhx1-iGFP in yeast cellsDepositorInsertNHX1 (NHX1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-ratCENP-C
Plasmid#174445Purposein vitro transcription template for rat CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-pumilioCENP-C
Plasmid#174446Purposein vitro transcription template for R. pumilio CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH858
Plasmid#172176PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH859
Plasmid#172177PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH580
Plasmid#172164PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2v2+B); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH750
Plasmid#172170PurposeMicroscopy inclusion body assayDepositorInsertpCDF.3f2, deleted sumo
UseTagsExpressionBacterialMutationEvolved PylBCD operon; SUMO tag deleted from PylB…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH751
Plasmid#172171PurposeMicroscopy inclusion body assayDepositorInsertpCDF.JM10.1, deleted sumo
UseTagsExpressionBacterialMutationEvolved PylBCD operon; SUMO tag deleted from PylB…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH768v2
Plasmid#172173PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH769v2
Plasmid#172174PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH191
Plasmid#172149PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*, Y184*); [strong promoter eG, strong RB…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only