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pRD411

Plasmid

#178196

Purpose

sRok overexpression

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

srok

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRD231

Plasmid

#178195

Purpose

Rok overexpression

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

rok

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S46C

Plasmid

#190008

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S46C

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S E63C

Plasmid

#190009

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, E63C

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_NT135

Plasmid

#183121

Purpose

pLentiGuide modified to express mCherry and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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InterTag-1xL

Plasmid

#186911

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)1-PDGFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB3

Plasmid

#167815

Purpose

Expresses His6-tagged AmmB3 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

AmmB3

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ammC1

Plasmid

#167816

Purpose

Expresses His6-tagged AmmC1 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

AmmC1

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEGPN

Plasmid

#187482

Purpose

Expression vector - GFP

Depositor

Lewis Cantley

Article

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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FAK C-term

Plasmid

#186147

Purpose

For expresseion of recombinant FAK c-terminal domain in E. coli

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pRoLuc

Plasmid

#135284

Purpose

GBPart - RoLuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

R. ohbai Luciferase

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMetLuc

Plasmid

#135286

Purpose

GBPart - MetLuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Metridia Luciferase

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-NHX1-iGFP

Plasmid

#177108

Purpose

Expresses Nhx1-iGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT-EGFP-ratCENP-C

Plasmid

#174445

Purpose

in vitro transcription template for rat CENP-C (N-ter EGFP tagged)

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Centromere protein C

Use

In vitro transcription template

Tags

EGFP

Expression

Mutation

Promoter

T7 SP6

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT-EGFP-pumilioCENP-C

Plasmid

#174446

Purpose

in vitro transcription template for R. pumilio CENP-C (N-ter EGFP tagged)

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Centromere protein C

Use

In vitro transcription template

Tags

EGFP

Expression

Mutation

Promoter

T7 SP6

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJH858

Plasmid

#172176

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH859

Plasmid

#172177

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH580

Plasmid

#172164

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2v2+B); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH750

Plasmid

#172170

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.3f2, deleted sumo

Use

Tags

Expression

Bacterial

Mutation

Evolved PylBCD operon; SUMO tag deleted from PylB…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH751

Plasmid

#172171

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.JM10.1, deleted sumo

Use

Tags

Expression

Bacterial

Mutation

Evolved PylBCD operon; SUMO tag deleted from PylB…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge