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Plasmid#185018PurposeshRNA mediated knockdownDepositorInsertERV_MMETn
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-CR-PA
Plasmid#175445PurposeHiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsBiP Secretion Peptide and Thrombin-Protein AExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:S495A
Plasmid#98151PurposeExpresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:S495A
UseTagsHis6-TEVExpressionBacterialMutationChanged serine 495 to alaninePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:W603A
Plasmid#98153PurposeExpresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:W603A
UseTagsHis6-TEVExpressionBacterialMutationChanged tryptophan 603 to alaninePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:H695Q
Plasmid#98154PurposeExpresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:H695Q
UseTagsHis6-TEVExpressionBacterialMutationChanged histidine 695 to glutaminePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:H695A
Plasmid#98155PurposeExpresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:H695A
UseTagsHis6-TEVExpressionBacterialMutationChanged histidine 695 to alaninePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:Y696G
Plasmid#98156PurposeExpresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:Y696G
UseTagsHis6-TEVExpressionBacterialMutationChanged tyrosine 696 to glycinePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZA3
Plasmid#158481PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationL17E, STOP codonPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA2
Plasmid#158480PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationD489V, STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA11
Plasmid#158489PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationL17E no STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA9
Plasmid#158487PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationno STOP codonPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA4
Plasmid#158482PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationL17E, D489V, STOP codonPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA10
Plasmid#158488PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationD489V, no STOP codonPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA12
Plasmid#158490PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationL17E, D489V, no STOP codonPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Clathrin-mGreenLantern
Plasmid#164462PurposeLocalization of mGreenLantern fluorescent protein to clathrinDepositorInsertmGreenLantern
UseTagsClathrinExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT-P2A-Clover-BlastR
Plasmid#138568PurposeUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJDepositorInsertP2A-clover-BlastR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
IMPT-10314:GABBR2
Plasmid#157851PurposeHA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expressionDepositorInsertGABBR2 (GABBR2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p(HA)HIF1alpha (P402A,P564A)
Plasmid#52636Purposemammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutationDepositorInsertHIF1alpha P402A, P564A (HIF1A Human)
UseTagsHAExpressionMammalianMutationP402A, P564APromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCSN067
Plasmid#101748PurposeSingle copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tubulin-mGreenLantern
Plasmid#164460PurposeLocalization of mGreenLantern fluorescent protein to tubulinDepositorInsertmGreenLantern
UseTagsTubulinExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only