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pDONR221-W279A-short-UBASH3A-Ctag

Plasmid

#195402

Purpose

Human W279A short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Gateway donor vector

Tags

Expression

Mutation

W279A

Promoter

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-K202R-short-UBASH3A-Ctag

Plasmid

#195399

Purpose

Human K202R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Gateway donor vector

Tags

Expression

Mutation

K202R

Promoter

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-2xGFP KI

Plasmid

#183443

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 2xGFP donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Kcnn2-smFP-myc KI

Plasmid

#183438

Purpose

FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP myc donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 GFP-Mpp2 KI

Plasmid

#183434

Purpose

CreOFF knock-in for GFP-MPP2 (amino acid position: A4)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 smFP-HA-Cacna1e KI

Plasmid

#183441

Purpose

FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Gria1-Halo KI

Plasmid

#183433

Purpose

CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-3xGFP KI

Plasmid

#183444

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 3xGFP donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 GFP-Actb KI

Plasmid

#183429

Purpose

CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Gria1-GFP KI

Plasmid

#183430

Purpose

CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-rScn2a-3xV5-Syn-miRFP670

Plasmid

#182566

Purpose

For rat Nav1.2 knockin with 3xV5 at the extracellular loop

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

rat Scn2a gRNA and 3xV5 donor DNA
miRFP670

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Syn and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66

Plasmid

#168787

Purpose

Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology; Expression in fila…

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-E2

Plasmid

#175243

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-Ter

Plasmid

#170634

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, Ter cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

Plasmid

#110408

Purpose

Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

none

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Strep II

Plasmid

#153512

Purpose

Strep II C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Strep II

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Aaw-Glucoamylase SP

Plasmid

#153497

Purpose

Aspergillus awamori Glucoamylase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Glucoamylase SP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Gga-Lysozym SP

Plasmid

#153505

Purpose

Chicken Lysozym Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Lysozym SP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TEV-His-GFP11

Plasmid

#153510

Purpose

TEV-His-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TEV-His-GFP11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Prepro SP

Plasmid

#153491

Purpose

S. cerevisiae Prepro Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Prepro SP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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