Showing: 1821 - 1840 of 4601 results
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pDN-D2irTNG4kwh
Plasmid#44722DepositorInsertspCMV-D2i promoter
htetR::NLS::EGFP
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailabilityAcademic Institutions and Nonprofits only -
hSyn Marina-T2A-nls-mCherry
Plasmid#85843Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertGEVI Marina
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterhSyn1AvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS
Plasmid#98566PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
ATP-dependent Clp protease adapter protein
UseTagsExpressionBacterialMutationIn a synthetic operon downstream of UBP1 and Remo…PromoterSame as UBP1 (operon) and Tet promoter (aTc induc…AvailabilityAcademic Institutions and Nonprofits only -
pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)
Plasmid#100596PurposepiggyBac vector expressing Dual-regulated Cas9 (Puro resistance)DepositorInsertsCRISPR Cas9 fused with human Glucocorticoid Receptor
mCherry
rtTA-M2
UsePiggybac vectorTagsExpressionMammalianMutationCodon-optimized for human codon usagePromoterDox-inducible TetO promoter and Human EEF1A1 prom…AvailabilityAcademic Institutions and Nonprofits only -
pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)
Plasmid#100597PurposepiggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)DepositorInsertsCRISPR Cas9 fused with human Glucocorticoid Receptor
mCherry
rtTA-M2
UsePiggybac vectorTagsExpressionMammalianMutationCodon-optimized for human codon usagePromoterDox-inducible TetO promoter and Human EEF1A1 prom…AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-HOIL-1 full-length (1–510)
Plasmid#193858PurposeExpression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coliDepositorInsertHOIL-1 (RBCK1 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationCodon optimised for expression in E. coliPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+FLAG-KrascomG12D
Plasmid#206844PurposeTo express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.DepositorInsertKras (Kras Mouse)
UseTagsFLAGExpressionMammalianMutationCodons altered to most optimum based on mouse cod…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBabePuro-FLAG-KrascomG12D
Plasmid#206862PurposeTo stably express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag in cells resistant to Neo..DepositorInsertKras (Kras Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationCodons in exons 1-3 altered to most optimum based…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBabePuro-FLAG-KrascomQ61R
Plasmid#206863PurposeTo stably express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag in cells resistant to Neo.DepositorInsertKras (Kras Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationCodons 1-3 altered to most optimum based on mouse…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pSR13
Plasmid#69155Purposeread-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr IIDepositorInsertsUseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0, codon-optimzed index 1.…Promoterrps-27 and swsn-1AvailabilityAcademic Institutions and Nonprofits only -
PARP1 FL
Plasmid#169815PurposeExpresses codon optimised full length His tagged PARP1 in bacterial cellsDepositorInsertPoly[ADP-ribose] polymerase 1 (PARP1 Human, Synthetic, Codon optimised)
UseTagsMKHHHHHHMKQExpressionBacterialMutationValine 762 mutated to an alaninePromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSB65 - pL0_tGFP-I (CDS1)
Plasmid#123182PurposeGolden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)DepositorInsertturboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
UsePart for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsPromoterAvailabilityAcademic Institutions and Nonprofits only -
peft-3::cas-9::tbb-2 3'UTR
Plasmid#48960Purposethis plasmid contains the codon optimized gene encoding Cas9 for C. elegans.DepositorInsertcas9
UseCRISPRTagsExpressionMutationPromotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
p201B Cas9
Plasmid#59177PurposeCas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
BAR
UseCRISPRTagsExpressionMutationPromoter2x35SAvailabilityAcademic Institutions and Nonprofits only -
p201G Cas9
Plasmid#59178PurposeCas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
sGFP
UseCRISPRTagsExpressionMutationPromoter2x35SAvailabilityAcademic Institutions and Nonprofits only -
pDUET-ctCPR-sAMO
Plasmid#20111DepositorInsertp450 reductase and codon optimized amorphadiene oxidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pMJ920
Plasmid#42234DepositorInsertCas9
UseCRISPRTagsGFP, HA epitope, and NLS (nuclear localization si…ExpressionMammalianMutationcodon-optimized synthetic DNA sequencePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI3
Plasmid#42527DepositorInsertWIPI3 (WDR45B Human)
UseTagsGST-TEVExpressionMammalianMutationgene sequence is codon optimized for expression i…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1821 - 1840 of 4601 results