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FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

Plasmid

#38262

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Use

Tags

Flag and PIP5K Iα core kinase domain (residues 18…

Expression

Mammalian

Mutation

Arg 233, Arg237, and Lys251 converted to Glutamin…

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
KLC1-Myc

Plasmid

#166962

Purpose

Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1 (Klc1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF1-MCS2-EF1-Puro-RGS7

Plasmid

#113728

Purpose

Express RGS7 in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX11-Flag-KAK

Plasmid

#120729

Purpose

Expression of a Flag-tagged version of DDX11 KAK mutant in mammalian cells

Depositor

Francesca Pisani

Article

Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

Insert

DDX11 KAK mutant (DDX11 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Substitution of E201, Y202 and E203 with K, A and…

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-delta 86-105

Plasmid

#78922

Purpose

Mammalian expression of RNF138 with delta 86-105 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta 86-105

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF138-delta 158-180

Plasmid

#78923

Purpose

Mammalian expression of RNF138 with delta 158-180 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta 159-180

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF138-delta 189-215

Plasmid

#78924

Purpose

Mammalian expression of RNF138 with delta 189-215 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta 189-215

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
HA-KIF5A

Plasmid

#166958

Purpose

Expresses HA-tagged KIF5A protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - HA-KLF4 FL delta K5S

Plasmid

#34594

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

deletion of K5S, corresponding to bases 1781 to 2…

Promoter

CMV

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
Lenti CGIP + M-AAT target

Plasmid

#86008

Purpose

lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
M-AAT target

Use

Lentiviral

Tags

Expression

Mutation

V30M

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-K5S-Mt344A

Plasmid

#34601

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-344 bi…

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S-Mt206B

Plasmid

#34600

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-206 bi…

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
GFP-VCF1 N167A

Plasmid

#223019

Purpose

Expression of GFP-tagged VCF1 (p97 binding-deficient N167A mutant) in mammalian cells.

Depositor

Niels Mailand

Article

Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

Insert

VCF1 (VCF1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

N167A

Promoter

CMV/TetO2

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Strep-HA-VCF1 WT

Plasmid

#223020

Purpose

Expression of Strep-HA-tagged VCF1 in mammalian cells.

Depositor

Niels Mailand

Article

Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

Insert

VCF1 (VCF1 Human)

Use

Tags

Strep-HA

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Strep-HA-VCF1 N167A

Plasmid

#223021

Purpose

Expression of Strep-HA-tagged VCF1 (p97 binding-deficient N167A mutant) in mammalian cells.

Depositor

Niels Mailand

Article

Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

Insert

VCF1 (VCF1 Human)

Use

Tags

Strep-HA

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-VCF1 WT

Plasmid

#223018

Purpose

Expression of GFP-tagged VCF1 in mammalian cells.

Depositor

Niels Mailand

Article

Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

Insert

VCF1 (VCF1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-scFVDnmt3L_bGHpA

Plasmid

#177356

Purpose

AAV expression of scFV-fused C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)

Use

AAV

Tags

myc and scFV

Expression

Mammalian

Mutation

194–415 aa

Promoter

CMV promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-Anillin deltaAnillin homology domain

Plasmid

#187277

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Anillin homology domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

deltaAnillin homology domain

Promoter

U6, CMV

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry-AH in pmCherryN1

Plasmid

#187284

Purpose

Express IL2-AH-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH

Promoter

CMV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry-AHA740D, E758K in pmCherryN1

Plasmid

#187285

Purpose

Express IL2-AH A740D, E758K-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH A740D, E758K

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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