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SPDK3214

Plasmid

#97406

Purpose

binary construct of ER-targeted GUS with sfCherry11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-GUS-sfCherry11

Use

Tags

ER target

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJOG545

Plasmid

#105424

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

AvrBs3

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI/ BpiI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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3xFNLDD/pCMV-7.1

Plasmid

#48874

Purpose

Expresses 3xFNLDD in mammalian cells

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

Insert

3xFNLDD

Use

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-ChBP

Plasmid

#108879

Purpose

Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-ChBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-5

Plasmid

#160705

Purpose

Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRC063

Plasmid

#123057

Purpose

Blue fluorescent protein

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

mTagBFP2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5910

Plasmid

#128717

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Insert

shcO-hopO1-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5058

Plasmid

#128710

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Insert

shcF-hopF2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Setd3-Flag-pGEX4t-1

Plasmid

#49145

Purpose

bacterial expression of GST-Setd3-Flag

Depositor

Jing H Wang

Article

Chen et al Haematologica. 2013 May;98(5):739-43. do

Insert

Setd3 (Setd3 Mouse)

Use

Tags

FLAG and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pMpGWB309

Plasmid

#68637

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pUPD-ADH2t (17-21)

Plasmid

#101701

Purpose

ADH2t in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GCTT-[terminator of S. cerevisiae YMR303C (376 bp)]-CGCT

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-PGK1 (01-11)

Plasmid

#101689

Purpose

pPGK1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YCR012W (982bp)]-CCAT

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCPP6534

Plasmid

#128735

Purpose

Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tag

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG387

Plasmid

#105338

Purpose

Synthetic biology; gateway destination vector assembly

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1018

Plasmid

#108975

Purpose

shuttle vector, N-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Expression

Mutation

Promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-NLS/HA-HumBeta (pSLIK2)

Plasmid

#113857

Purpose

For Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase gene

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

Humanized Beta

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

"humanized" Beta gene

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJOG562

Plasmid

#105340

Purpose

Synthetic biology; gateway destination vector assembly

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNb-delta S

Plasmid

#107984

Purpose

bacterial expression of OPN splice variant b

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNb 1038bp-17aa signal (SPP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

WT splice variant

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-OPNc-delta S

Plasmid

#107985

Purpose

bacterial expression of OPN splice variant c

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

OPNc 999bp-16aa signal (SPP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

WT splice variant

Promoter

Available since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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mCitrine-USP19 C506S

Plasmid

#78594

Purpose

Express mCitrine-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 C506S (USP19 Human)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only