160,747 results

1. Synapsin sHRPa-NRX in FSW lentiviral vector

   Plasmid

   #73147

   Purpose

   Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-NRX
   

   Use

   Lentiviral

   Tags

   2x HA tag and Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNK497

   Plasmid

   #219754

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-SpRY-His

   Plasmid

   #179320

   Purpose

   Plasmid for bacterial expression and purification of SpRY-Cas9

   Depositor

   Julián Cerón, Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Human codon-optimized Cas9 variant SpRY
   

   Use

   CRISPR

   Tags

   6xHis and SV40-NLS

   Expression

   Bacterial

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBacPAK8-2xHA-MOF

   Plasmid

   #29763

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   MYST1 (KAT8 Human)
   

   Use

   Tags

   2xHA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

   Plasmid

   #158967

   Purpose

   Ubiquitous expression of mtON in C. elegans

   Depositor

   Andrew Wojtovich

   Article

   Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

   Insert

   eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   eft-3

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET-RCaMP1h

   Plasmid

   #42874

   Purpose

   a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

   Depositor

   Loren Looger

   Article

   Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

   Insert

   Red Fluorescent Calcium binding Protein
   

   Use

   Tags

   EK (Enterokinase) Recognition Site, His Tag (6x),…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1+ SMN1 myc HIS

   Plasmid

   #71687

   Purpose

   Mammalian expression of SMN1

   Depositor

   Gideon Dreyfuss

   Insert

   SMN1 (SMN1 Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSGH2

   Plasmid

   #186706

   Purpose

   an artificial heat-inducible promoter

   Depositor

   Thomas Czerny, Baubak Bajoghli

   Article

   Bajoghli et al Dev Biol. 2004 Jul 15;271(2):416-30. doi

   Inserts

   GFP
   luc
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

   Plasmid

   #197504

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-ABE8e-NRCH-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCP-ADAR2-DD(E488Q)

    Plasmid

    #170125

    Purpose

    Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD(E488Q)-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHen2 Avi-tag-linker-p3

    Plasmid

    #119819

    Purpose

    For cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    linker with Avi-tag and p3
    

    Use

    Tags

    Avi-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

    Plasmid

    #31481

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce target) EF BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry contains M9S and M16L to reduce backgroun…

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IP-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #84572

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMito-Electra2

    Plasmid

    #184927

    Purpose

    Mitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8A

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    COX8A-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. RSV-Flag-Brd2

    Plasmid

    #86614

    Purpose

    Flag tagged mouse Brd2 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd2 (Brd2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV GG hUbC-dsRED

    Plasmid

    #84034

    Purpose

    Contains Golden Gate cassette to express up to four gRNAs and dsRED

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. -962 human cyclin D1 promoter pGL3Basic

    Plasmid

    #32727

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase; Luciferase reporter gene

    Tags

    Expression

    Mutation

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. KU70-eGFP

    Plasmid

    #213517

    Purpose

    Expresses human KU70-eGFP in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    human KU70-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAP05

    Plasmid

    #102920

    Purpose

    Direct cloning and broad-host-range heterologous expression vector

    Depositor

    Bradley Moore

    Article

    Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. hPGK_V5-APEX2-GFP

    Plasmid

    #105285

    Purpose

    drives expression of V5-NES-APEX2-GFP from human PGK promotor

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    V5-APEX2-NES
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK promoter

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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