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Plasmid#225154PurposeMedium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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pC0.388
Plasmid#203940PurposeUp Flank. Neutral site. Up flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.DepositorInsertmrr UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.389
Plasmid#203941PurposeDown Flank. Neutral site. Down flanking sequence of restriction endonuclease Mrr (FEK30_09380) in Synechococcus sp. PCC 11901.DepositorInsertmrr DOWN
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-HR1bR181A R185A-GFP-L (B3-B4)
Plasmid#211857PurposeMutant E3-DART degron casette for N-terminal fusion to protein of interest using GoldenbraidDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionMutationR181A R185APromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
β2AR-PPI-CALPon1.0 (TEVp)
Plasmid#213407PurposePeroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)DepositorInsertHA-arrestin-TEVp
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIGII-TCR-F
Plasmid#194392PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIGII-TCR-G
Plasmid#194393PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIAII-TCR-A
Plasmid#194394PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIAII-TCR-B
Plasmid#194395PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIAII-TCR-C
Plasmid#194396PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIAII-TCR-D
Plasmid#194397PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIAII-TCR-E
Plasmid#194398PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIAII-TCR-F
Plasmid#194399PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIAII-TCR-G
Plasmid#194400PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIGII-TCR-A
Plasmid#194387PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIGII-TCR-B
Plasmid#194388PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIGII-TCR-C
Plasmid#194389PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIGII-TCR-D
Plasmid#194390PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIGII-TCR-E
Plasmid#194391PurposeRetroviral expression of candidate orphan TCRDepositorUseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNGsacB
Plasmid#203676PurposepQFT with mNeonGreen and sacB under control of PQJDepositorInsertsmNeonGreen
sacB
UseTagsExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGI…PromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only