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Plasmid#86278PurposeDonor vector for 3' FLAG tag of human ZNF580DepositorInsertZNF580 homology arms (ZNF580 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_SAP130
Plasmid#86267PurposeDonor vector for 3' FLAG tag of human SAP130DepositorInsertSAP130 homology arms (SAP130 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAWV
Plasmid#62710PurposeDonor vector for amiR-eGFP knock-in via landing padDepositorInserttdTomato/amiR-eGFP123/amiR-eGFP419/FLPe
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223_NKX2-1_DelCTerm_1-223AA
Plasmid#221476PurposeGateway cloning of NKX2-1 C-terminal truncation mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 224-371AA C-terminal domainPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_DelNTerm_160-371AA
Plasmid#221475PurposeGateway cloning of NKX2-1 N-terminal truncation mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal domainPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rif1-Halo HRD
Plasmid#207081PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF168-Halo HRD
Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
UseTagsExpressionMammalianMutationPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF169-Halo HRD
Plasmid#207083PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.DepositorInsertHaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-Sec61B
Plasmid#207553PurposeHomologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-Sec61B
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SNAP-Sec61B
Plasmid#207580PurposeHomologous recombination donor to integrate and expression cassette for SNAPtag-Sec61B in the AAVS1 locus of human cellsDepositorInsertTET inducible SNAPTag-Sec61B
UseTagsSNAPTAGExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 LAMP1-mNeonGreen HRD
Plasmid#207549PurposeHomologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cellsDepositorInsertTET inducible LAMP1-mNeonGreen
UseTagsmNeonGreenExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-LC3B HRD
Plasmid#207551PurposeHomologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-LC3B
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-GABARAPL1 HRD
Plasmid#207552PurposeHomologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-GABARAPL1
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
SNAP-LC3 HRD
Plasmid#207545PurposeHomologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cellsDepositorInsertSNAPtag with internal PuroR cassette flanked by human LC3B locus sequences
UseTagsSNAPtagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG5 HRD
Plasmid#207542PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG5 locus in human cellsDepositorInsertHaloTag flanked by human ATG5 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-LC3B HRD
Plasmid#207539PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cellsDepositorInsertHaloTag with internal PuroR cassette flanked by human LC3B locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only