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pAAV-UbC-eGFP

Plasmid

#62518

Purpose

Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.

Depositor

Pantelis Tsoulfas, Jae Lee

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1A-WT-Flag-UTR

Plasmid

#183237

Purpose

Expression of FLAG tagged HNF1A

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox A (HNF1A Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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HEpl017

Plasmid

#179992

Purpose

pCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

TadA8e-dSiCas12i(D1049A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

Plasmid

#187276

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

deltaActin binding domain

Promoter

U6, CMV

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

Plasmid

#187275

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

deltaMyosin binding domain

Promoter

U6, CMV

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec22b-P33-shR

Plasmid

#208359

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations (shRNA resistance), 33 aa p…

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 R313PRR

Plasmid

#184629

Purpose

Mammalian expression vector with N-terminal Flag for KBTBD4 R313PRR expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 R313PRR (KBTBD4 Human)

Use

Tags

Expression

Mammalian

Mutation

R313PRR

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 P311PP

Plasmid

#184628

Purpose

Mammalian expression vector with N-terminal 3xFlag for KBTBD4 P311PP expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 P311PP (KBTBD4 Human)

Use

Tags

Expression

Mammalian

Mutation

P311PP

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 R312RG

Plasmid

#184630

Purpose

Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)

Use

Tags

Expression

Mammalian

Mutation

R312RG

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-KLC1

Plasmid

#166964

Purpose

Expresses Myc-tagged KLC1 protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1 (Klc1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIX-Myc-hTIM3

Plasmid

#110893

Purpose

Expression of Myc-tagged hTIM3 at the cell surface

Depositor

Hyeryun Choe

Article

Jemielity et al PLoS Pathog. 2013 Mar;9(3):e1003232. doi

Insert

Hepatitis A virus Cellular Receptor 2 (HAVCR2) (HAVCR2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

CMV

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBT272_(pRosa26-GTET)

Plasmid

#36882

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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px330-CIB1-dCas9

Plasmid

#63665

Purpose

Mammalian gRNA expression vector also expressing CIB1-dCas9

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and CMV

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5A

Plasmid

#166954

Purpose

Expresses FLAG-tagged KIF5A protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR

Plasmid

#196518

Purpose

Expression of Ctdnep1_D67E-GFP

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1_D67EsiR (CTDNEP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

D67E mutation (phosphastase dead). Silent mutatio…

Promoter

CMV promoter; T7 promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PCAF (delta5-53a.a.)

Plasmid

#65388

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PCAF (KAT2B Human)

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

lost 13-159bp of the ORF

Promoter

CMV

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-DDX11-Flag-KAE

Plasmid

#120728

Purpose

Expression of a Flag-tagged version of DDX11 KAE mutant in mammalian cells

Depositor

Francesca Pisani

Article

Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

Insert

DDX11 KAE mutant (DDX11 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Substitution of E201 and Y202 with K and A

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-delta 18-58

Plasmid

#78921

Purpose

Mammalian expression of RNF138 with delta 18-58 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta 18-58

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Flag-KLF6-4D (1054)

Plasmid

#49490

Purpose

expresses human Flag tagged KLF6 with 4D mutation

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

KFL6-4D (KLF6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

three serines and one tyrosine mutated to aspart…

Promoter

CMV

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
flag-hKLF6-4A (1010)

Plasmid

#49489

Purpose

expresses human Flag tagged KLF6 with 4A mutation

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

KFL6-4A (KLF6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

three serines and one tyrosine mutated to alanin…

Promoter

CMV

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only