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Plasmid#51408PurposeNon-PI(4,5)P2 binding control for PH-PLCD1-GFPDepositorInsertPH-PLCD1 (aa 1ā170) (PLCD1 Human)
UseTagsEGFPExpressionMammalianMutationArginine 40 mutated to leucinePromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk-GFP
Plasmid#51463PurposeIs a biosensor for PIP3DepositorInsertBTK (aa 1ā177) (BTK Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
PM-FRB-CFP
Plasmid#67517PurposeFRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)DepositorInsertmTOR (MTOR Human)
UseTagsCFP, HA, and PM tethering sequence (MLCCMRRTKQVEKā¦ExpressionMammalianMutationFRB domain only (residues 2019ā2114)PromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
PM2-FRB-CFP
Plasmid#67518PurposeFRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)DepositorInsertmTOR (MTOR Human)
UseTagsCFP, HA, and PM tethering sequence from human Lynā¦ExpressionMammalianMutationFRB domain only (residues 2019ā2114)PromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk(R28C)-GFP
Plasmid#51464PurposeNon-PIP3 binding control for PH-Btk-GFPDepositorInsertBTK (aa 1ā177) (BTK Human)
UseTagsGFPExpressionMammalianMutationR28CPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP12-5ptpase domain
Plasmid#67516PurposeRecruitable human type IV 5-phosphatase domain (214-644)DepositorUseTagsmRFPExpressionMutationINPP5E has C641A to destroy the C-terminal CAAX dā¦PromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP12-5ptpase enzyme
Plasmid#67515PurposeRecruitable human type IV 5-phosphatase enzymeDepositorUseTagsmRFPExpressionMammalianMutationINPP5E has C641A to destroy the C-terminal CAAX dā¦PromoterCMVAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCELF4-4405
Plasmid#115466PurposeConstitutive lentiviral expressionDepositorInsertshCELF4-4405 (CELF4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
shYAP1 # 1
Plasmid#42540DepositorInsertYAP1 (YAP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
shYAP1 # 2
Plasmid#42541DepositorInsertYAP1 (YAP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro shKRAS
Plasmid#116871PurposeTet-inducible suppression of KRASDepositorInsertshKRAS (KRAS Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1/TOAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
shBCL2L1 # 2
Plasmid#42552DepositorInsertshBCL2L1 (BCL2L1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
shBCL2L1 # 1
Plasmid#42551DepositorInsertshBCL2L1 (BCL2L1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
shCTNNB1 # 2
Plasmid#42544DepositorInsertCTNNB1 (CTNNB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
shCTNNB1 # 1
Plasmid#42543DepositorInsertCTNNB1 (CTNNB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
shBIRC5 # 2
Plasmid#42554DepositorInsertshBIRC5 (BIRC5 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
shBIRC5 # 1
Plasmid#42553DepositorInsertshBIRC5 (BIRC5 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCELF4-4404
Plasmid#115465PurposeConstitutive lentiviral expressionDepositorInsertshCELF4-4404 (CELF4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG
Plasmid#69114PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationL1939A and L1942A amino acid substitutions. Sileā¦PromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.neo_shGFP
Plasmid#110470PurposeControl, silence GFP gene, doxycycline inducible, neomycin selectionDepositorInsertGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1/TO (RNA PolIII)Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only