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Plasmid#124128PurposeBacterial expression plasmid for PURE system componentsDepositorInsertElongation factor Ts (tsf Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-NDK
Plasmid#124136PurposeBacterial expression plasmid for PURE system componentsDepositorInsertNucleotide diphosphate kinase (ndk Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-AspRS-His
Plasmid#124106PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAspartate-tRNA ligase (aspS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE60-RRF-His
Plasmid#124132PurposeBacterial expression plasmid for PURE system componentsDepositorInsertRibosome recycling factor (frr Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LysRS-His
Plasmid#124114PurposeBacterial expression plasmid for PURE system componentsDepositorInsertLysine-tRNA ligase (lysU Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His-RF2
Plasmid#124130PurposeBacterial expression plasmid for PURE system componentsDepositorInsertRelease factor 2 (prfB Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-ProRS-His
Plasmid#124117PurposeBacterial expression plasmid for PURE system componentsDepositorInsertProlyl-tRNA ligase (proS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-His-IleRS
Plasmid#124112PurposeBacterial expression plasmid for PURE system componentsDepositorInsertIsoleucyl-tRNA ligase (ileS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TrpRS-His
Plasmid#124120PurposeBacterial expression plasmid for PURE system componentsDepositorInsertTryptophanyl-tRNA ligase (trpS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TyrRS-His
Plasmid#124121PurposeBacterial expression plasmid for PURE system componentsDepositorInsertTyrosyl-tRNA ligase (tyrS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-ValRS-His
Plasmid#124122PurposeBacterial expression plasmid for PURE system componentsDepositorInsertValyl-tRNA ligase (valS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LeuRS-His
Plasmid#124113PurposeBacterial expression plasmid for PURE system componentsDepositorInsertLeucyl-tRNA ligase (leuS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-HisRS-His
Plasmid#124111PurposeBacterial expression plasmid for PURE system componentsDepositorInsertHistidyl-tRNA ligase (hisS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GluRS-His
Plasmid#124109PurposeBacterial expression plasmid for PURE system componentsDepositorInsertGlutamyl-tRNA ligase (gltX Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorInsertCysteinyl-tRNA ligase (cysS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_neomycin
Plasmid#65308PurposeLuminal ER hook only (RUSH system)DepositorInsertInvariant chain (p33)
UseTagsExpressionMammalianMutationStreptavidin in its lumenal partPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
VSVGwt-SBP-EGFP_puromycin
Plasmid#65302PurposeVSVG reporter only (no hook) (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_VSVG-SBP-EGFP
Plasmid#65300Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST44-AP-1 core
Plasmid#184809PurposeBacterial expression of clathrin adaptor AP-1 core complexDepositorInsertHs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
UseTagsGST tag and His6ExpressionBacterialMutationAP1S3: S148C; AP1B1: K359R, E476KPromoterT7Available sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_neomycin
Plasmid#65306PurposeLuminal ER hook only (RUSH system)DepositorInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
UseTagsKDEL motif and Signal SequenceExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only